OAS1 antibody (Middle Region)

Catalog No. ABIN2773987

There are 2+ publications for this product available. The Rabbit Polyclonal anti-OAS1 antibody is suitable to detect OAS1 in samples from Human, Mouse, Rat, Cow, Dog, Pig, Horse, Rabbit and Guinea Pig. It has been validated for WB and IHC.

Quick Overview for OAS1 antibody (Middle Region) (ABIN2773987)

Target: OAS1 (2',5'-Oligoadenylate Synthetase 1, 40/46kDa (OAS1))

Reactivity: Human, Mouse, Rat, Cow, Dog, Pig, Horse, Rabbit, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This OAS1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-OAS1 Antibody

Binding Specificity: Middle Region

Purpose: OAS1 Antibody - middle region

Sequence: RRQLTKPRPV ILDPADPTGN LGGGDPKGWR QLAQEAEAWL NYPCFKNWDG

Predicted Reactivity: Cow: 93%, Dog: 93%, Guinea Pig: 87%, Horse: 93%, Human: 100%, Mouse: 93%, Pig: 93%, Rabbit: 86%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against OAS1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human OAS1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP51359-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-OAS1 antibody (ABIN2773987)

Yu, Yan, Liu, Li, Liu, Zhu, Chen, Han: "Pattern recognition receptor-initiated innate antiviral response in mouse adipose cells." in: Immunology and cell biology, Vol. 92, Issue 2, pp. 105-15, (2014) (PubMed).

Zhu, Chen, Yan, Liu, Li, Zhang, Yu, Chen, Han: "RIG-I-like receptors mediate innate antiviral response in mouse testis." in: Molecular endocrinology (Baltimore, Md.), Vol. 27, Issue 9, pp. 1455-67, (2013) (PubMed).

Target Details for OAS1

Target: OAS1 (2',5'-Oligoadenylate Synthetase 1, 40/46kDa (OAS1))

Alternative Name: OAS1

Background:

Background Information: This protein is a member of the 2-5A synthetase family, essential proteins involved in the innate immune response to viral infection. The encoded protein is induced by interferons and uses adenosine triphosphate in 2'-specific nucleotidyl transfer reactions to synthesize 2',5'-oligoadenylates (2-5As). These molecules activate latent RNase L, which results in viral RNA degradation and the inhibition of viral replication.This gene encodes a member of the 2-5A synthetase family, essential proteins involved in the innate immune response to viral infection. The encoded protein is induced by interferons and uses adenosine triphosphate in 2'-specific nucleotidyl transfer reactions to synthesize 2',5'-oligoadenylates (2-5As). These molecules activate latent RNase L, which results in viral RNA degradation and the inhibition of viral replication. The three known members of this gene family are located in a cluster on chromosome 12. Mutations in this gene have been associated with host susceptibility to viral infection. Alternatively spliced transcript variants encoding different isoforms have been described.

Gene Name: 2'-5'-oligoadenylate synthetase 1, 40/46 kDa

Alternative Symbols: OIAS, IFI-4, OIASI, E18/E16

Protein Name: 2'-5'-oligoadenylate synthase 1

Molecular Weight: 46 kDa

Gene ID: 4938

NCBI Accession: NP_001027581

UniProt: P00973

Pathways: Hepatitis C