CHN2 antibody (N-Term)

Catalog No. ABIN2773996

The Rabbit Polyclonal anti-CHN2 antibody is suitable to detect CHN2 in samples from Human, Mouse, Rat, Cow, Horse, Rabbit, Dog and Guinea Pig. It has been validated for WB.

Quick Overview for CHN2 antibody (N-Term) (ABIN2773996)

Target: CHN2 (Chimerin (Chimaerin) 2 (CHN2))

Reactivity: Human, Mouse, Rat, Cow, Horse, Rabbit, Dog, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHN2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CHN2 Antibody

Binding Specificity: N-Term

Purpose: CHN2 Antibody - N-terminal region

Sequence: AFGVKVGVKG GFLWPPLKLF ACSQISSLVR RAALTHNDNH FNYEKTHNFK

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against CHN2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human CHN2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8096889

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for CHN2

Target: CHN2 (Chimerin (Chimaerin) 2 (CHN2))

Alternative Name: CHN2

Background:

Background Information: CHN2 is a protein with a phorbol-ester/DAG-type zinc finger, a Rho-GAP domain and an SH2 domain. This protein has GTPase-activating protein activity that is regulated by phospholipid binding and binding of diacylglycerol (DAG) induces translocation of the protein from the cytosol to the Golgi apparatus membrane. CHN2 plays a role in the proliferation and migration of smooth muscle cells. Decreased expression of this gene is associated with high-grade gliomas and breast tumors, and increased expression of this gene is associated with lymphomas. Mutations in this gene have been associated with schizophrenia in men.This gene is a member of the chimerin family and encodes a protein with a phorbol-ester/DAG-type zinc finger, a Rho-GAP domain and an SH2 domain. This protein has GTPase-activating protein activity that is regulated by phospholipid binding and binding of diacylglycerol (DAG) induces translocation of the protein from the cytosol to the Golgi apparatus membrane. The protein plays a role in the proliferation and migration of smooth muscle cells. Decreased expression of this gene is associated with high-grade gliomas and breast tumors, and increased expression of this gene is associated with lymphomas. Mutations in this gene have been associated with schizophrenia in men. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Gene Name: Chimerin (chimaerin) 2

Alternative Symbols: BCH, CHN2-3, ARHGAP3, RHOGAP3

Protein Name: Beta-chimaerin

Molecular Weight: 38kDa

Gene ID: 1124

NCBI Accession: NP_001035025

UniProt: P52757