WARS2 antibody (Middle Region)

Catalog No. ABIN2774013

Cited in 1 publication. The Rabbit Polyclonal anti-WARS2 antibody (ABIN2774013) specifically detects WARS2 in WB. The antibody is reactive with Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Saccharomyces cerevisiae and Zebrafish (Danio rerio) samples.

Quick Overview for WARS2 antibody (Middle Region) (ABIN2774013)

Target: WARS2 (Tryptophanyl tRNA Synthetase 2, Mitochondrial (WARS2))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Saccharomyces cerevisiae, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WARS2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-WARS2 Antibody

Binding Specificity: Middle Region

Purpose: WARS2 Antibody - middle region

Sequence: TTKQKHDGTV GLLTYPVLQA ADILLYKSTH VPVGEDQVQH MELVQDLAQG

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 93%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against WARS2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human WARS2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8114844

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-WARS2 antibody (ABIN2774013)

Wang, Sips, Khin, Rotival, Sun, Ahmed, Widjaja, Schafer, Yusoff, Choksi, Ko, Singh, Epstein, Guan, Houštěk, Mracek, Nuskova, Mikell, Tan, Pesce, Kolar, Bottolo, Mancini, Hubner, Pravenec, Petretto et al.: "Wars2 is a determinant of angiogenesis. ..." in: Nature communications, Vol. 7, pp. 12061, (2016) (PubMed).

Target Details for WARS2

Target: WARS2 (Tryptophanyl tRNA Synthetase 2, Mitochondrial (WARS2))

Alternative Name: WARS2

Background:

Background Information: Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of tryptophanyl-tRNA synthetase exist, a cytoplasmic form, named WARS, and a mitochondrial form, named WARS2. WARS2 is the mitochondrial tryptophanyl-tRNA synthetase. Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of tryptophanyl-tRNA synthetase exist, a cytoplasmic form, named WARS, and a mitochondrial form, named WARS2. This gene encodes the mitochondrial tryptophanyl-tRNA synthetase. Two alternative transcripts encoding different isoforms have been described.

Gene Name: Tryptophanyl tRNA synthetase 2, mitochondrial

Alternative Symbols: TrpRS, NEMMLAS, mtTrpRS

Protein Name: Tryptophan--tRNA ligase, mitochondrial

Molecular Weight: 24kDa

Gene ID: 10352

NCBI Accession: NP_957715

UniProt: Q9UGM6