GCLC antibody (N-Term)

Catalog No. ABIN2774078

The Rabbit Polyclonal anti-GCLC antibody has been validated for WB and IHC. It is suitable to detect GCLC in samples from Human, Mouse, Rat, Cow, Zebrafish (Danio rerio), Rabbit, Dog, Guinea Pig and Horse. There is 1 publication available.

Quick Overview for GCLC antibody (N-Term) (ABIN2774078)

Target: GCLC (Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC))

Reactivity: Human, Mouse, Rat, Cow, Zebrafish (Danio rerio), Rabbit, Dog, Guinea Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GCLC antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-GCLC Antibody

Binding Specificity: N-Term

Sequence: VLETLQEKGE RTNPNHPTLW RPEYGSYMIE GTPGQPYGGT MSEFNTVEAN

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 93%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 79%

Characteristics: This is a rabbit polyclonal antibody against GCLC. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human GCLC

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 637 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-GCLC antibody (ABIN2774078)

Tomasi, Ryoo, Yang, Iglesias Ara, Ko, Lu: "Molecular mechanisms of lipopolysaccharide-mediated inhibition of glutathione synthesis in mice." in: Free radical biology & medicine, Vol. 68, pp. 148-58, (2014) (PubMed).

Target Details for GCLC

Target: GCLC (Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC))

Alternative Name: GCLC

Background: Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. GCLC is the catalytic subunit of 637 amino acids with a calculated molecular weight of 72.773 kDa. Deficiency of gamma-glutamylcysteine synthetase in human is associated with enzymopathic hemolytic anemia.Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. The gene encoding the catalytic subunit encodes a protein of 367 amino acids with a calculated molecular weight of 72.773 kDa and maps to chromosome 6. The regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Deficiency of gamma-glutamylcysteine synthetase in human is associated with enzymopathic hemolytic anemia. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: GCS, GLCL, GLCLC, GCL
Protein Interaction Partner: UBC, PPID, GCLM, CCL22, PAXIP1, ELAVL1,
Protein Size: 637

Molecular Weight: 73 kDa

Gene ID: 2729

NCBI Accession: NM_001498, NP_001489

UniProt: P48506

Pathways: Cell RedoxHomeostasis