MASA antibody (N-Term)

Catalog No. ABIN2774190

The Rabbit Polyclonal anti-MASA antibody is suitable to detect MASA in samples from Human, Mouse, Rat, Cow, Dog, Horse, Rabbit and Guinea Pig. It has been validated for WB.

Quick Overview for MASA antibody (N-Term) (ABIN2774190)

Target: MASA (ENOPH1) (Enolase-Phosphatase 1 (ENOPH1))

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Rabbit, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MASA antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MASA Antibody

Binding Specificity: N-Term

Purpose: ENOPH1 Antibody - N-terminal region

Sequence: IEENVKEYLQ THWEEEECQQ DVSLLRKQAE EDAHLDGAVP IPAASGNGVD

Predicted Reactivity: Cow: 93%, Dog: 93%, Guinea Pig: 100%, Horse: 79%, Human: 100%, Mouse: 100%, Rabbit: 93%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against ENOPH1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ENOPH1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP57529-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for MASA

Target: MASA (ENOPH1) (Enolase-Phosphatase 1 (ENOPH1))

Alternative Name: ENOPH1

Background:

Background Information: ENOPH1 is a bifunctional enzyme that catalyzes the enolization of 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P) into the intermediate 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate (HK-MTPenyl-1-P), which is then dephosphorylated to form the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene).

Gene Name: Enolase-phosphatase 1

Alternative Symbols: E1, MASA, mtnC, MST145

Protein Name: Enolase-phosphatase E1

Molecular Weight: 29kDa

Gene ID: 58478

NCBI Accession: NP_067027

UniProt: Q9UHY7

Pathways: Methionine Biosynthetic Process