UBA1 antibody (N-Term)

Catalog No. ABIN2774233

There is 1 publication for this product available. The Rabbit Polyclonal anti-UBA1 antibody is suitable to detect UBA1 in samples from Human, Mouse, Rat, Dog, Cow, Horse, Rabbit and Guinea Pig. It has been validated for WB.

Quick Overview for UBA1 antibody (N-Term) (ABIN2774233)

Target: UBA1 (Ubiquitin-Like Modifier Activating Enzyme 1 (UBA1))

Reactivity: Human, Mouse, Rat, Dog, Cow, Horse, Rabbit, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBA1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-UBA1 Antibody

Binding Specificity: N-Term

Purpose: UBA1 Antibody - N-terminal region

Sequence: MEAGPPGSAR PAEPGPCLSG QRGADHTASA SLQSVAGTEP GRHPQAVAAV

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against UBA1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human UBA1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP58687-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-UBA1 antibody (ABIN2774233)

Rogers, Olivier, Meyerholz, Dupuy: "Adaptive immunity does not strongly suppress spontaneous tumors in a Sleeping Beauty model of cancer." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 190, Issue 8, pp. 4393-9, (2013) (PubMed).

Target Details for UBA1

Target: UBA1 (Ubiquitin-Like Modifier Activating Enzyme 1 (UBA1))

Alternative Name: UBA1

Background:

Background Information: UBA1 activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thioester and free AMP.The protein encoded by this gene catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. This gene complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair. It is part of a gene cluster on chromosome Xp11.23. Alternatively spliced transcript variants that encode the same protein have been described.

Gene Name: Ubiquitin-like modifier activating enzyme 1

Alternative Symbols: A1S9, A1ST, GXP1, UBE1, A1S9T, AMCX1, POC20, SMAX2, UBA1A, UBE1X, VEXAS, CFAP124

Protein Name: Ubiquitin-like modifier-activating enzyme 1

Molecular Weight: 116kDa

Gene ID: 7317

NCBI Accession: NP_003325

UniProt: P22314