MRPS16 antibody (C-Term)

Catalog No. ABIN2774462

The Rabbit Polyclonal anti-MRPS16 antibody is suitable to detect MRPS16 in samples from Human, Rat and Guinea Pig. It has been validated for WB.

Quick Overview for MRPS16 antibody (C-Term) (ABIN2774462)

Target: MRPS16 (Mitochondrial Ribosomal Protein S16 (MRPS16))

Reactivity: Human, Rat, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MRPS16 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MRPS16 Antibody

Binding Specificity: C-Term

Purpose: MRPS16 Antibody - C-terminal region

Sequence: EKLLGLAGFF PLHPMMITNA ERLRRKRARE VLLASQKTDA EATDTEATET

Predicted Reactivity: Guinea Pig: 75%, Human: 100%, Rat: 75%

Characteristics: This is a rabbit polyclonal antibody against MRPS16. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C-terminal region of Human MRPS16

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP66866-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for MRPS16

Target: MRPS16 (Mitochondrial Ribosomal Protein S16 (MRPS16))

Alternative Name: MRPS16

Background:

Background Information: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75 % protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S16P family. The encoded protein is one of the most highly conserved ribosomal proteins between mammalian and yeast mitochondria. Three pseudogenes (located at 8q21.3, 20q13.32, 22q12-q13.1) for this gene have been described.

Alternative Symbols: COXPD2, RPMS16, CGI-132, MRP-S16

Protein Name: 28S ribosomal protein S16, mitochondrial

Molecular Weight: 15kDa

Gene ID: 51021

NCBI Accession: NP_057149

UniProt: Q9Y3D3