THOC7 antibody (C-Term)

Catalog No. ABIN2774475

The Rabbit Polyclonal anti-THOC7 antibody is suitable to detect THOC7 in samples from Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit and Zebrafish (Danio rerio). It has been validated for WB.

Quick Overview for THOC7 antibody (C-Term) (ABIN2774475)

Target: THOC7 (THO Complex 7 (THOC7))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This THOC7 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-THOC7 Antibody

Binding Specificity: C-Term

Purpose: THOC7 Antibody - C-terminal region

Sequence: ELEHLSHIKE SVEDKLELRR KQFHVLLSTI HELQQTLEND EKLSEVEEAQ

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 86%, Rabbit: 100%, Rat: 100%, Zebrafish: 90%

Characteristics: This is a rabbit polyclonal antibody against THOC7. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C-terminal region of Human THOC7

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP67323-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for THOC7

Target: THOC7 (THO Complex 7 (THOC7))

Alternative Name: THOC7

Background:

Targeted Subunit: 7 homolog

Background Information: THOC7 is a component of the THO subcomplex of the TREX complex. The TREX complex specifically associates with spliced mRNA and not with unspliced pre-mRNA. It is recruited to spliced mRNAs by a transcription-independent mechanism. Binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export. The recruitment occurs via an interaction between ALYREF/THOC4 and the cap-binding protein NCBP1. DDX39B functions as a bridge between ALYREF/THOC4 and the THO complex. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. The recruitment of the TREX complex to the intronless viral mRNA occurs via an interaction between KSHV ORF57 protein and ALYREF/THOC4.

Alternative Symbols: fSAP24, hTREX30, NIF3L1BP1

Protein Name: THO complex subunit 7 homolog

Molecular Weight: 22kDa

Gene ID: 80145

NCBI Accession: NP_079351

UniProt: Q6I9Y2