CXCL12 antibody (Middle Region)

Catalog No. ABIN2774605

There is 1 publication for this product available. The Rabbit Polyclonal anti-CXCL12 antibody is suitable to detect CXCL12 in samples from Rat, Dog, Cow and Pig. It has been validated for WB.

Quick Overview for CXCL12 antibody (Middle Region) (ABIN2774605)

Target: CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))

Reactivity: Rat, Dog, Cow, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CXCL12 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CXCL12 Antibody

Binding Specificity: Middle Region

Purpose: CXCL12 Antibody - middle region

Sequence: VKHLKILNTP NCALQIVARL KNNNRQVCID PKLKWIQEYL EKALNKRFKM

Predicted Reactivity: Cow: 90%, Dog: 100%, Pig: 100%, Rat: 90%

Characteristics: This is a rabbit polyclonal antibody against CXCL12. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human CXCL12

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-CXCL12 antibody (ABIN2774605)

Yin, Seeberger: "Applications of heparin and heparan sulfate microarrays." in: Methods in enzymology, Vol. 478, pp. 197-218, (2010) (PubMed).

Target Details for CXCL12

Target: CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))

Alternative Name: CXCL12

Background:

Background Information: Stromal cell-derived factors 1-alpha and 1-beta are small cytokines that belong to the intercrine family, members of which activate leukocytes and are often induced by proinflammatory stimuli such as lipopolysaccharide, TNF, or IL1. The intercrines are characterized by the presence of 4 conserved cysteines which form 2 disulfide bonds. They can be classified into 2 subfamilies. In the CC subfamily, which includes beta chemokine, the cysteine residues are adjacent to each other. In the CXC subfamily, which includes alpha chemokine, they are separated by an intervening amino acid. The SDF1 proteins belong to the latter group.

Gene Name: Chemokine (C-X-C motif) ligand 12

Alternative Symbols: IRH, PBSF, SDF1, TLSF, TPAR1, SCYB12

Protein Name: Stromal cell-derived factor 1

Molecular Weight: 11 kDa

Gene ID: 6387

NCBI Accession: NP_000600

UniProt: P48061

Pathways: Regulation of Cell Size, CXCR4-mediated Signaling Events, Negative Regulation of intrinsic apoptotic Signaling