DMRT1 antibody (N-Term)

Catalog No. ABIN2774657

The Rabbit Polyclonal anti-DMRT1 antibody has been validated for WB. It is suitable to detect DMRT1 in samples from Human, Cow, Dog, Rabbit and Pig.

Quick Overview for DMRT1 antibody (N-Term) (ABIN2774657)

Target: DMRT1 (Doublesex and Mab-3 Related Transcription Factor 1 (DMRT1))

Reactivity: Human, Cow, Dog, Rabbit, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DMRT1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-DMRT1 Antibody

Binding Specificity: N-Term

Sequence: PNDEAFSKPS TPSEAPHAPG VPPQGRAGGF GKASGALVGA ASGSSAGGSS

Cross-Reactivity: Cow (Bovine), Dog (Canine), Human, Pig (Porcine)

Predicted Reactivity: Cow: 78%, Dog: 85%, Human: 100%, Pig: 78%, Rabbit: 92%

Characteristics: This is a rabbit polyclonal antibody against DMRT1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human DMRT1

Application Details

Application Notes: WB Suggested Anti-DMRT1 Antibody Titration: 0.2-1 µg/mL
Positive Control: Human brain.
Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 373 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for DMRT1

Target: DMRT1 (Doublesex and Mab-3 Related Transcription Factor 1 (DMRT1))

Alternative Name: DMRT1

Background: The gene encodes the DMRT1 protein is found in a cluster with two other members of the gene family, having in common a zinc finger-like DNA-binding motif (DM domain). The DM domain is an ancient, conserved component of the vertebrate sex-determining pathway that is also a key regulator of male development in flies and nematodes. This gene exhibits a gonad-specific and sexually dimorphic expression pattern. Defective testicular development and XY feminization occur when this gene is hemizygous. This suggested that DMRT1 may be required for testis development.This gene is found in a cluster with two other members of the gene family, having in common a zinc finger-like DNA-binding motif (DM domain). The DM domain is an ancient, conserved component of the vertebrate sex-determining pathway that is also a key regulator of male development in flies and nematodes. This gene exhibits a gonad-specific and sexually dimorphic expression pattern. Defective testicular development and XY feminization occur when this gene is hemizygous. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: DMT1
Protein Interaction Partner: Dlg4, TRIM29,
Protein Size: 373

Molecular Weight: 39 kDa

Gene ID: 1761

NCBI Accession: NM_021951, NP_068770

UniProt: Q9Y5R6