FLI1 antibody (Middle Region)

Catalog No. ABIN504666

This anti-FLI1 antibody is a Rabbit Polyclonal antibody detecting FLI1 in WB and IHC. Suitable for Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit and Zebrafish (Danio rerio).

Quick Overview for FLI1 antibody (Middle Region) (ABIN504666)

Target: FLI1 (Friend Leukemia Virus Integration 1 (FLI1))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FLI1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-FLI1 Antibody

Binding Specificity: Middle Region

Sequence: FDFHGIAQAL QPHPTESSMY KYPSDISYMP SYHAHQQKVN FVPPHPSSMP

Cross-Reactivity: Cow (Bovine), Chicken, Human, Mouse (Murine), Rat (Rattus)

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 85%, Human: 100%, Mouse: 92%, Rabbit: 100%, Rat: 92%, Zebrafish: 92%

Characteristics: This is a rabbit polyclonal antibody against FLI1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human FLI1

Application Details

Application Notes: WB Suggested Anti-FLI1 Antibody Titration: 0.2-1 µg/mL
ELISA Titer: 1:312500
Positive Control: Jurkat cell lysate.
Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 452 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for FLI1

Target: FLI1 (Friend Leukemia Virus Integration 1 (FLI1))

Alternative Name: FLI1

Background: Friend leukemia integration 1 (Fli-1) is a member of the ETS family of transcriptional regulatory proteins that contain a highly conserved and structurally unique DNA binding ETS domain.
Alias Symbols: EWSR2, SIC-1
Protein Interaction Partner: SOX30, SIAH1, CTBP2, UBC, SMAD9, GATA1, FMNL1, SUMO1, UBE2I, PIAS2, PIAS1, SMAD3, ETV6, KLF1, TDP2, SRF, ERG, EWSR1, ELK1, BRCA1, TGFBR2, STYXL1,
Protein Size: 452

Molecular Weight: 51 kDa

Gene ID: 2313

NCBI Accession: NM_002017, NP_002008

UniProt: Q01543