FBXW2 antibody (Middle Region)

Catalog No. ABIN2774691

Cited in 1 publication. The Rabbit Polyclonal anti-FBXW2 antibody (ABIN2774691) specifically detects FBXW2 in WB. The antibody is reactive with Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit and Zebrafish (Danio rerio) samples.

Quick Overview for FBXW2 antibody (Middle Region) (ABIN2774691)

Target: FBXW2 (F-Box and WD Repeat Domain Containing 2 (FBXW2))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FBXW2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-FBXW2 Antibody

Binding Specificity: Middle Region

Purpose: FBXW2 Antibody - middle region

Sequence: SLISRWPLPE YRKSKRGSSF LAGEASWLNG LDGHNDTGLV FATSMPDHSI

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against FBXW2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human FBXW2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP43109-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-FBXW2 antibody (ABIN2774691)

Van Rechem, Black, Abbas, Allen, Rinehart, Yuan, Dutta, Whetstine: "The SKP1-Cul1-F-box and leucine-rich repeat protein 4 (SCF-FbxL4) ubiquitin ligase regulates lysine demethylase 4A (KDM4A)/Jumonji domain-containing 2A (JMJD2A) protein." in: The Journal of biological chemistry, Vol. 286, Issue 35, pp. 30462-70, (2011) (PubMed).

Target Details for FBXW2

Target: FBXW2 (F-Box and WD Repeat Domain Containing 2 (FBXW2))

Alternative Name: FBXW2

Background:

Background Information: F-box proteins are an expanding family of eukaryotic proteins characterized by an approximately 40 amino acid motif, the F box. Some F-box proteins have been shown to be critical for the ubiquitin-mediated degradation of cellular regulatory proteins. In fact, F-box proteins are one of the four subunits of ubiquitin protein ligases, called SCFs. SCF ligases bring ubiquitin conjugating enzymes to substrates that are specifically recruited by the different F-box proteins. Mammalian F-box proteins are classified into three groups based on the presence of either WD-40 repeats, leucine-rich repeats, or the presence or absence of other protein-protein interacting domains. FBXW2 is the second identified member of the F-box family and contains multiple WD-40 repeats.F-box proteins are an expanding family of eukaryotic proteins characterized by an approximately 40 amino acid motif, the F box. Some F-box proteins have been shown to be critical for the ubiquitin-mediated degradation of cellular regulatory proteins. In fact, F-box proteins are one of the four subunits of ubiquitin protein ligases, called SCFs. SCF ligases bring ubiquitin conjugating enzymes to substrates that are specifically recruited by the different F-box proteins. Mammalian F-box proteins are classified into three groups based on the presence of either WD-40 repeats, leucine-rich repeats, or the presence or absence of other protein-protein interacting domains. This gene encodes the second identified member of the F-box gene family and contains multiple WD-40 repeats.

Gene Name: F-box and WD repeat domain containing 2

Alternative Symbols: Md6, FBW2, Fwd2

Protein Name: F-box/WD repeat-containing protein 2

Molecular Weight: 51kDa

Gene ID: 26190

NCBI Accession: NP_036296

UniProt: Q9UKT8