UBE3C antibody (N-Term)

Catalog No. ABIN2774719

The Rabbit Polyclonal anti-UBE3C antibody is suitable to detect UBE3C in samples from Human, Mouse, Rat, Cow, Guinea Pig, Rabbit, Zebrafish (Danio rerio) and Dog. It has been validated for WB.

Quick Overview for UBE3C antibody (N-Term) (ABIN2774719)

Target: UBE3C (Ubiquitin Protein Ligase E3C (UBE3C))

Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Rabbit, Zebrafish (Danio rerio), Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE3C antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-UBE3C Antibody

Binding Specificity: N-Term

Purpose: UBE3C Antibody - N-terminal region

Sequence: MFSFEGDFKT RPKVSLGGAS RKYSIQRSAF DRCATLSQSG GAFPIANGPN

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against UBE3C. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N-terminal region of Human UBE3C

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP43163-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for UBE3C

Target: UBE3C (Ubiquitin Protein Ligase E3C (UBE3C))

Alternative Name: UBE3C

Background:

Background Information: E3 ubiquitin-protein ligase that accepts ubiquitin from the E2 ubiquitin-conjugating enzyme UBE2D1 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. UBE3C can assemble unanchored poly-ubiquitin chains in either 'Lys-29'- or 'Lys-48'-linked polyubiquitin chains. It has preference for 'Lys-48' linkages. It can target itself for ubiquitination in vitro and may promote its own degradation in vivo.

Gene Name: ubiquitin protein ligase E3C

Alternative Symbols: HECTH2

Protein Name: Ubiquitin-protein ligase E3C

Molecular Weight: 44kDa

Gene ID: 9690

UniProt: Q15386