RNF43 antibody (Middle Region)

Catalog No. ABIN2774743

There is 1 publication for this product available. The Rabbit Polyclonal anti-RNF43 antibody is suitable to detect RNF43 in samples from Human, Mouse, Rat, Cow, Dog, Horse and Rabbit. It has been validated for WB.

Quick Overview for RNF43 antibody (Middle Region) (ABIN2774743)

Target: RNF43 (Ring Finger Protein 43 (RNF43))

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RNF43 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-RNF43 Antibody

Binding Specificity: Middle Region

Purpose: RNF43 Antibody - middle region

Sequence: DFDPLVYCSP KGDPQRVDMQ PSVTSRPRSL DSVVPTGETQ VSSHVHYHRH

Predicted Reactivity: Cow: 86%, Dog: 93%, Horse: 93%, Human: 100%, Mouse: 100%, Rabbit: 93%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against RNF43. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human RNF43

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP43227-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-RNF43 antibody (ABIN2774743)

Sugiura, Yamaguchi, Miyamoto: "A cancer-associated RING finger protein, RNF43, is a ubiquitin ligase that interacts with a nuclear protein, HAP95." in: Experimental cell research, Vol. 314, Issue 7, pp. 1519-28, (2008) (PubMed).

Target Details for RNF43

Target: RNF43 (Ring Finger Protein 43 (RNF43))

Alternative Name: RNF43

Background:

Background Information: RNF43 is a HAP95 (AKAP8L) binding ubiquitin ligase that promotes cell growth and is upregulated in colon cancer.

Gene Name: Ring finger protein 43

Alternative Symbols: URCC, SSPCS, RNF124

Protein Name: E3 ubiquitin-protein ligase RNF43

Molecular Weight: 86 kDa

Gene ID: 54894

NCBI Accession: NP_060233

UniProt: Q68DV7

Pathways: WNT Signaling