HERC3 antibody (Middle Region)

Catalog No. ABIN2774751

This Rabbit Polyclonal antibody specifically detects HERC3 in WB. It exhibits reactivity toward Human, Mouse, Rat, Guinea Pig, Rabbit, Cow, Dog, Horse, Saccharomyces cerevisiae and Zebrafish (Danio rerio).

Quick Overview for HERC3 antibody (Middle Region) (ABIN2774751)

Target: HERC3 (Hect Domain and RLD 3 (HERC3))

Reactivity: Human, Mouse, Rat, Guinea Pig, Rabbit, Cow, Dog, Horse, Saccharomyces cerevisiae, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HERC3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-HERC3 Antibody

Binding Specificity: Middle Region

Purpose: HERC3 Antibody - middle region

Sequence: LHFPLALYKK LLNVKPGLED LKELSPTEGR SLQELLDYPG EDVEETFCLN

Predicted Reactivity: Cow: 92%, Dog: 100%, Guinea Pig: 100%, Horse: 92%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 91%, Zebrafish: 85%

Characteristics: This is a rabbit polyclonal antibody against HERC3. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human HERC3

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP43238-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for HERC3

Target: HERC3 (Hect Domain and RLD 3 (HERC3))

Alternative Name: HERC3

Background:

Background Information: This gene encodes a member the HERC ubiquitin ligase family. The encoded protein is located in the cytosol and binds ubiquitin via a HECT domain. Mutations in this gene have been associated with colorectal and gastric carcinomas. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Gene Name: HECT and RLD domain containing E3 ubiquitin protein ligase 3

Protein Name: probable E3 ubiquitin-protein ligase HERC3

Molecular Weight: 54kDa

Gene ID: 8916

NCBI Accession: NP_001258531

UniProt: Q15034