MARCH1 antibody (N-Term)

Catalog No. ABIN2774752

Product Details anti-MARCH1 Antibody

Target: MARCH1 (Membrane-Associated Ring Finger (C3HC4) 1, E3 Ubiquitin Protein Ligase (MARCH1))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Cow, Horse, Rabbit, Guinea Pig, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MARCH1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: LQASSPTTGT APRSQSRLSV CPSTQDICRI CHCEGDEESP LITPCRCTGT

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against MARCH1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human MARCH1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 272 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for MARCH1

Target: MARCH1 (Membrane-Associated Ring Finger (C3HC4) 1, E3 Ubiquitin Protein Ligase (MARCH1))

Alternative Name: MARCH1 (MARCH1 Products)

Synonyms: MARCH-I antibody, RNF171 antibody, 2900024D24Rik antibody, BB085186 antibody, RGD1305148 antibody, MARCH1 antibody, DKFZp459K1250 antibody, membrane associated ring-CH-type finger 1 antibody, membrane-associated ring finger (C3HC4) 1 antibody, MARCH1 antibody, March1 antibody

Background: MARCH1 is an E3 ubiquitin-protein ligase that may mediate ubiquitination of TFRC, CD86 and FAS, and promote their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.
Alias Symbols: DKFZp564M1682, FLJ20668, MARCH-I, RNF171
Protein Interaction Partner: TNFRSF10A, UBE2H, UBE2D1, CDC34, TFRC, UBC, HLA-DRB1, HLA-DRA, PPP2R1B, PPP2R1A, CD86,
Protein Size: 272

Molecular Weight: 31 kDa

Gene ID: 55016

NCBI Accession: NM_017923, NP_060393