SH3RF1 antibody (Middle Region)

Catalog No. ABIN2774771

Product Details anti-SH3RF1 Antibody

Target: SH3RF1 (SH3 Domain Containing Ring Finger 1 (SH3RF1))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Guinea Pig, Horse, Rabbit, Cow, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SH3RF1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: LLKLLSGAST KRKPRVSPPA SPTLEVELGS AELPLQGAVG PELPPGGGHG

Predicted Reactivity: Cow: 93%, Dog: 93%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against SH3RF1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human SH3RF1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 888 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for SH3RF1

Target: SH3RF1 (SH3 Domain Containing Ring Finger 1 (SH3RF1))

Alternative Name: SH3RF1 (SH3RF1 Products)

Synonyms: SH3MD2 antibody, posh antibody, xPOSH antibody, rnf142 antibody, sh3md2 antibody, MGC135632 antibody, MGC189605 antibody, POSH antibody, RNF142 antibody, 2200003J05Rik antibody, Posh antibody, R75531 antibody, Sh3md2 antibody, si:ch211-114c9.2 antibody, si:dkey-15j16.4 antibody, wu:fi42f11 antibody, SH3 domain containing ring finger 1 antibody, SH3 domain containing ring finger 1 S homeolog antibody, SH3RF1 antibody, sh3rf1 antibody, sh3rf1.S antibody, Sh3rf1 antibody

Background: SH3RF1 acts as a scaffold protein, contributes to Rac-induced signal transduction such as JNKs (MAPK8 and MAPK9) activation and induces apoptosis. Within a signaling complex, it probably recruits protein kinases such as MAP3K10 or MAP3K11 which are in turn activated leading to the sequential activation of MAP2K4, MAP2K7 and JNKs (MAPK8 and MAPK9). SH3RF1 may be involved in targeting of HIV-1 GAG and GAG-POL polyproteins to the plasma membrane.This gene encodes a protein containing an N-terminus RING-finger, four SH3 domains, and a region implicated in binding of the Rho GTPase Rac. Via the RING-finger, the encoded protein has been shown to function as an ubiquitin-protein ligase involved in protein sorting at the trans-Golgi network. The encoded protein may also act as a scaffold for the c-Jun N-terminal kinase signaling pathway, facilitating the formation of a functional signaling module.
Alias Symbols: FLJ21602, KIAA1494, POSH, RNF142, SH3MD2
Protein Interaction Partner: YWHAB, UBE2N, UBE2D3, UBE2D1, AKT2, AKT1, SIAH2, SIAH1, SH3RF2, RAC1, SEMA6A, UBC, PDCD6IP, KCNJ1, MAP4K3, MAP3K9, ADAM15, PAK2, RACGAP1, MAP3K12, MAP3K10, MAP3K11,
Protein Size: 888

Molecular Weight: 93 kDa

Gene ID: 57630

NCBI Accession: NM_020870, NP_065921

UniProt: Q7Z6J0