MARCH7 antibody (Middle Region)

Catalog No. ABIN2774777

The Rabbit Polyclonal anti-MARCH7 antibody (ABIN2774777) specifically detects MARCH7 in WB. The antibody is reactive with Human, Mouse, Rat, Cow, Dog, Horse, Rabbit and Guinea Pig samples.

Quick Overview for MARCH7 antibody (Middle Region) (ABIN2774777)

Target: MARCH7 (Membrane-Associated Ring Finger (C3HC4) 7, E3 Ubiquitin Protein Ligase (MARCH7))

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Rabbit, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MARCH7 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MARCH7 Antibody

Binding Specificity: Middle Region

Sequence: SSMSSTFFSR RSSQDSLNTR SLNSENSYVS PRILTASQSR SNVPSASEVP

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against MARCH7. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human MARCH7

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 704 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for MARCH7

Target: MARCH7 (Membrane-Associated Ring Finger (C3HC4) 7, E3 Ubiquitin Protein Ligase (MARCH7))

Alternative Name: MARCH7

Background: MARCH7 is an E3 ubiquitin-protein ligase which may specifically enhance the E2 activity of HIP2. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.
Alias Symbols: AXO, AXOT, DKFZP586F1122, MARCH-VII, RNF177
Protein Interaction Partner: MARCH7, UBE2M, UBE2N, UBE2E1, UBE2D3, UBE2D2, UBE2D1, PPP3CB, PPP3CA, MAPT, UBC, UBE2K, USP7, TRIM65, RNF111, USP9X, VAV2,
Protein Size: 704

Molecular Weight: 78 kDa

Gene ID: 64844

NCBI Accession: NM_022826, NP_073737

UniProt: Q9H992