ITCH antibody (N-Term)

Catalog No. ABIN2774796

The Rabbit Polyclonal anti-ITCH antibody has been validated for WB. It is suitable to detect ITCH in samples from Human, Rat, Cow, Rabbit, Dog, Horse and Guinea Pig.

Quick Overview for ITCH antibody (N-Term) (ABIN2774796)

Target: ITCH (Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH))

Reactivity: Human, Rat, Cow, Rabbit, Dog, Horse, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ITCH antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ITCH Antibody

Binding Specificity: N-Term

Sequence: QLESEVVTNG ETTCSESASQ NDDGSRSKDE TRVSTNGSDD PEDAGAGENR

Predicted Reactivity: Cow: 86%, Dog: 92%, Guinea Pig: 93%, Horse: 86%, Human: 100%, Rabbit: 93%, Rat: 86%

Characteristics: This is a rabbit polyclonal antibody against ITCH. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ITCH

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 862 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for ITCH

Target: ITCH (Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH))

Alternative Name: ITCH

Background: Atrophin-1 contains a polyglutamine repeat, expansion of which is responsible for dentatorubral and pallidoluysian atrophy. The protein encoded by this gene interacts with atrophin-1. This encoded protein is a closely related member of the NEDD4-like prot
Alias Symbols: AIF4, AIP4, NAPP1, dJ468O1.1
Protein Interaction Partner: GSN, UBE2L3, UBC, ITCH, WWOX, GGA3, UBE2D2, PTCH1, ATXN3, HTT, EGFR, SMAD6, DTX3L, CXCR4, LATS1, POLR2A, HNRNPL, EWSR1, RPAP2, CPSF7, RPAP3, UBAP2, PABPC1, DAZAP1, WBP2, SMARCE1, SMARCC2, SMARCC1, POLR2E, POLR2C, SPG20, HNRNPUL1, UBAP2L, URI1, ARID1A, SF1
Protein Size: 862

Molecular Weight: 99 kDa

Gene ID: 83737

NCBI Accession: NM_031483, NP_113671

UniProt: A9Z1X3

Pathways: Activation of Innate immune Response, CXCR4-mediated Signaling Events