ITCH antibody (N-Term)

Catalog No. ABIN2774796

Product Details anti-ITCH Antibody

Target: ITCH (Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH))

Binding Specificity: N-Term

Reactivity: Human, Rat, Cow, Rabbit, Dog, Horse, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ITCH antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: QLESEVVTNG ETTCSESASQ NDDGSRSKDE TRVSTNGSDD PEDAGAGENR

Predicted Reactivity: Cow: 86%, Dog: 92%, Guinea Pig: 93%, Horse: 86%, Human: 100%, Rabbit: 93%, Rat: 86%

Characteristics: This is a rabbit polyclonal antibody against ITCH. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ITCH

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 862 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for ITCH

Target: ITCH (Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH))

Alternative Name: ITCH (ITCH Products)

Background: Atrophin-1 contains a polyglutamine repeat, expansion of which is responsible for dentatorubral and pallidoluysian atrophy. The protein encoded by this gene interacts with atrophin-1. This encoded protein is a closely related member of the NEDD4-like prot
Alias Symbols: AIF4, AIP4, NAPP1, dJ468O1.1
Protein Interaction Partner: GSN, UBE2L3, UBC, ITCH, WWOX, GGA3, UBE2D2, PTCH1, ATXN3, HTT, EGFR, SMAD6, DTX3L, CXCR4, LATS1, POLR2A, HNRNPL, EWSR1, RPAP2, CPSF7, RPAP3, UBAP2, PABPC1, DAZAP1, WBP2, SMARCE1, SMARCC2, SMARCC1, POLR2E, POLR2C, SPG20, HNRNPUL1, UBAP2L, URI1, ARID1A, SF1
Protein Size: 862

Molecular Weight: 99 kDa

Gene ID: 83737

NCBI Accession: NM_031483, NP_113671

UniProt: A9Z1X3

Pathways: Activation of Innate immune Response, CXCR4-mediated Signaling Events