NUCB2 antibody (Middle Region)

Catalog No. ABIN2774832

The Rabbit Polyclonal anti-NUCB2 antibody has been validated for WB and IHC. It is suitable to detect NUCB2 in samples from Human, Rat, Mouse, Dog, Horse, Cow, Pig, Rabbit, Guinea Pig and Saccharomyces cerevisiae. There are 2+ publications available.

Quick Overview for NUCB2 antibody (Middle Region) (ABIN2774832)

Target: NUCB2 (Nucleobindin 2 (NUCB2))

Reactivity: Human, Rat, Mouse, Dog, Horse, Cow, Pig, Rabbit, Guinea Pig, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NUCB2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-NUCB2 Antibody

Binding Specificity: Middle Region

Sequence: MMKEHERREY LKTLNEEKRK EEESKFEEMK KKHENHPKVN HPGSKDQLKE

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 86%, Horse: 100%, Human: 100%, Mouse: 93%, Pig: 100%, Rabbit: 93%, Rat: 93%, Yeast: 91%

Characteristics: This is a rabbit polyclonal antibody against NUCB2. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human NUCB2

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 420 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-NUCB2 antibody (ABIN2774832)

Takagi, Miki, Tanaka, Hashimoto, Watanabe, Sasano, Ito, Suzuki: "Nucleobindin 2 (NUCB2) in human endometrial carcinoma: a potent prognostic factor associated with cell proliferation and migration." in: Endocrine journal, Vol. 63, Issue 3, pp. 287-99, (2016) (PubMed).

Suzuki, Takagi, Miki, Onodera, Akahira, Ebata, Ishida, Watanabe, Sasano, Suzuki: "Nucleobindin 2 in human breast carcinoma as a potent prognostic factor." in: Cancer science, Vol. 103, Issue 1, pp. 136-43, (2012) (PubMed).

Target Details for NUCB2

Target: NUCB2 (Nucleobindin 2 (NUCB2))

Alternative Name: NUCB2

Background: Nucleobindin-2 is a calcium-binding EF-hand protein.
Alias Symbols: NEFA
Protein Interaction Partner: UBC, NEDD8, TUBB6, CAMK1D, NAE1, HSPA1L, CLU, GNAS, GNAI3, GADD45A, XPO1, SMARCD1, CUL2, NR1I2, NDN, STAR,
Protein Size: 420

Molecular Weight: 46 kDa

Gene ID: 4925

NCBI Accession: NM_005013, NP_005004

UniProt: P80303