Annexin VII antibody (N-Term)

Catalog No. ABIN2774843

Cited in 1 publication. The Rabbit Polyclonal anti-Annexin VII antibody (ABIN2774843) specifically detects Annexin VII in WB. The antibody is reactive with Human, Mouse, Rat, Cow, Rabbit, Dog, Guinea Pig and Horse samples.

Quick Overview for Annexin VII antibody (N-Term) (ABIN2774843)

Target: Annexin VII (ANXA7) (Annexin A7 (ANXA7))

Reactivity: Human, Mouse, Rat, Cow, Rabbit, Dog, Guinea Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Annexin VII antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Annexin VII Antibody

Binding Specificity: N-Term

Purpose: ANXA7 Antibody - N-terminal region

Sequence: MSYPGYPPTG YPPFPGYPPA GQESSFPPSG QYPYPSGFPP MGGGAYPQVP

Predicted Reactivity: Cow: 86%, Dog: 93%, Guinea Pig: 93%, Horse: 93%, Human: 100%, Mouse: 86%, Rabbit: 93%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against ANXA7. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ANXA7

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8094740

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-Annexin VII antibody (ABIN2774843)

Satoh, Nakano, Shibata, Maki: "The penta-EF-hand domain of ALG-2 interacts with amino-terminal domains of both annexin VII and annexin XI in a Ca2+-dependent manner." in: Biochimica et biophysica acta, Vol. 1600, Issue 1-2, pp. 61-7, (2002) (PubMed).

Target Details for Annexin VII

Target: Annexin VII (ANXA7) (Annexin A7 (ANXA7))

Alternative Name: ANXA7

Background:

Background Information: ANXA7 is a member of the annexin family of calcium-dependent phospholipid binding proteins. The Annexin VII gene contains 14 exons and spans approximately 34 kb of DNA. An alternatively spliced cassette exon results in two mRNA transcripts of 2.0 and 2.4 kb which are predicted to generate two protein isoforms differing in their N-terminal domain. The alternative splicing event is tissue specific and the mRNA containing the cassette exon is prevalent in brain, heart and skeletal muscle. The transcripts also differ in their 3'-non coding regions by the use of two alternative poly(A) signals. The selection of poly(A) signals is independent of the mRNA splicing pattern. Annexin VII encodes a protein with a molecular weight of approximately 51 kDa with a unique, highly hydrophobic N-terminal domain of 167 amino acids and a conserved C-terminal region of 299 amino acids. The latter domain is composed of alternating hydrophobic and hydrophilic segments. Structural analysis of the protein suggests that Annexin VII is a membrane binding protein with diverse properties including voltage-sensitive calcium channel activity, ion selectivity and membrane fusion.

Gene Name: Annexin A7

Alternative Symbols: SNX, ANX7, SYNEXIN

Protein Name: Annexin A7

Molecular Weight: 51kDa

Gene ID: 310

NCBI Accession: NP_001147

UniProt: Q5T0M6