MCM3 antibody (C-Term)

Catalog No. ABIN2774880

This Rabbit Polyclonal antibody specifically detects MCM3 in WB and IHC. It exhibits reactivity toward Human, Mouse, Rat, Horse, Pig, Cow, Guinea Pig, Rabbit and Dog.

Quick Overview for MCM3 antibody (C-Term) (ABIN2774880)

Target: MCM3 (Minichromosome Maintenance Complex Component 3 (MCM3))

Reactivity: Human, Mouse, Rat, Horse, Pig, Cow, Guinea Pig, Rabbit, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MCM3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-MCM3 Antibody

Binding Specificity: C-Term

Sequence: SQEDQEQKRK RRKTRQPDAK DGDSYDPYDF SDTEEEMPQV HTPKTADSQE

Predicted Reactivity: Cow: 92%, Dog: 93%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 92%, Pig: 100%, Rabbit: 93%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against MCM3. It was validated on Western Blot and immunohistochemistry.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human MCM3

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 808 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for MCM3

Target: MCM3 (Minichromosome Maintenance Complex Component 3 (MCM3))

Alternative Name: MCM3

Background: MCM3 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with, and thus is acetlyated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression.The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with, and thus is acetlyated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression.
Alias Symbols: HCC5, MGC1157, P1-MCM3, P1.h, RLFB
Protein Interaction Partner: SUMO2, MCMBP, UBC, KEAP1, MDM2, MCM6, MCM5, ACTBL2, MGEA5, CAP1, SAE1, HSP90B1, TPM3, HSP90AA1, FKBP5, FKBP4, MIB1, YWHAQ, UBD, TCEA1, MCM10, ORC5, ORC4, MCM2, ITGA4, FN1, CSNK2A1, CDK2, CDC6, CDC5L, PNKP, CDC7, VCAM1, NOTCH1, POLD1, POLA1, MCM7, PIPSL, P
Protein Size: 808

Molecular Weight: 89 kDa

Gene ID: 4172

NCBI Accession: NM_002388, NP_002379

UniProt: P25205

Pathways: DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA