MCM5 antibody (N-Term)

Catalog No. ABIN2774889

This Rabbit Polyclonal antibody specifically detects MCM5 in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Rabbit and Horse.

Quick Overview for MCM5 antibody (N-Term) (ABIN2774889)

Target: MCM5 (Minichromosome Maintenance Complex Component 5 (MCM5))

Reactivity: Human, Mouse, Rat, Cow, Rabbit, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MCM5 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MCM5 Antibody

Binding Specificity: N-Term

Sequence: MSGFDDPGIF YSDSFGGDAQ ADEGQARKSQ LQRRFKEFLR QYRVGTDRTG

Predicted Reactivity: Cow: 86%, Horse: 86%, Human: 100%, Mouse: 79%, Rabbit: 86%, Rat: 79%

Characteristics: This is a rabbit polyclonal antibody against MCM5. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human MCM5

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 734 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for MCM5

Target: MCM5 (Minichromosome Maintenance Complex Component 5 (MCM5))

Alternative Name: MCM5

Background: The protein encoded by MCM5 is structurally very similar to the CDC46 protein from S. cerevisiae, a protein involved in the initiation of DNA replication. The encoded protein is a member of the MCM family of chromatin-binding proteins and can interact with at least two other members of this family. The encoded protein is upregulated in the transition from the G0 to G1/S phase of the cell cycle and may actively participate in cell cycle regulation.
Alias Symbols: CDC46, P1-CDC46
Protein Interaction Partner: KRTAP10-7, RBPMS, SUMO2, SUMO3, MCMBP, UBC, RNF2, NEK9, MCM3, HSP90AB1, HSP90AA1, FKBP5, MAGED2, CAP1, BAG3, CD81, IGSF8, TCEA1, PNKP, VCAM1, ORC5, ORC1, MCM7, MCM2, ITGA4, FN1, CDKN2A, CDC5L, NOTCH1, MYBBP1A, U2AF1, MCM6, MCM4, HNRNPU, APP, FBXO6, NEDD8,
Protein Size: 734

Molecular Weight: 82 kDa

Gene ID: 4174

NCBI Accession: NM_006739, NP_006730

UniProt: P33992

Pathways: DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA