MCM4 antibody (C-Term)

Catalog No. ABIN2774894

This anti-MCM4 antibody is a Rabbit Polyclonal antibody detecting MCM4 in WB. Suitable for Human, Mouse, Rat, Rabbit, Cow, Guinea Pig, Dog, Horse and Zebrafish (Danio rerio).

Quick Overview for MCM4 antibody (C-Term) (ABIN2774894)

Target: MCM4 (Minichromosome Maintenance Deficient 4 (MCM4))

Reactivity: Human, Mouse, Rat, Rabbit, Cow, Guinea Pig, Dog, Horse, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MCM4 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MCM4 Antibody

Binding Specificity: C-Term

Sequence: KEELAEALKK LILSKGKTPA LKYQQLFEDI RGQSDIAITK DMFEEALRAL

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 93%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against MCM4. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human MCM4

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 863 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for MCM4

Target: MCM4 (Minichromosome Maintenance Deficient 4 (MCM4))

Alternative Name: MCM4

Background: The protein encoded by MCM4 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. This gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. Alternatively spliced transcript variants encoding the same protein have been reported.
Alias Symbols: CDC21, CDC54, hCdc21, P1-CDC21
Protein Interaction Partner: HUWE1, UBC, PCNA, SUMO2, FKBP5, MCMBP, MDM2, POLK, CLUH, NCKAP1, CAP1, CAP2, EIF3B, MCM2, KPNA3, PRMT1, FKBP4, MCM7, MCM6, FBXW4, FBXO46, ITGA4, CDC5L, gag-pol, ORC6, CCNA1, VCAM1, DBF4, CDC7, LIG4, MCM5, MCM3, DCTN2, SF3A1, UBD, NEDD8, TONSL, SIRT7, ASF1
Protein Size: 863

Molecular Weight: 95 kDa

Gene ID: 4173

NCBI Accession: NM_182746, NP_877423

UniProt: P33991

Pathways: DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA