AKAP10 antibody (Middle Region)

Catalog No. ABIN2775175

The Rabbit Polyclonal anti-AKAP10 antibody has been validated for WB. It is suitable to detect AKAP10 in samples from Human, Rat, Mouse, Rabbit, Cow, Dog, Horse, Pig and Zebrafish (Danio rerio).

Quick Overview for AKAP10 antibody (Middle Region) (ABIN2775175)

Target: AKAP10 (A Kinase (PRKA) Anchor Protein 10 (AKAP10))

Reactivity: Human, Rat, Mouse, Rabbit, Cow, Dog, Horse, Pig, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AKAP10 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-AKAP10 Antibody

Binding Specificity: Middle Region

Sequence: ESLYQRTYAG KMTFGRVSDL GQFIRESEPE PDVRKSKGSM FSQAMKKWVQ

Predicted Reactivity: Cow: 93%, Dog: 93%, Horse: 93%, Human: 100%, Mouse: 93%, Pig: 93%, Rabbit: 93%, Rat: 93%, Zebrafish: 90%

Characteristics: This is a rabbit polyclonal antibody against AKAP10. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human AKAP10

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 662 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for AKAP10

Target: AKAP10 (A Kinase (PRKA) Anchor Protein 10 (AKAP10))

Alternative Name: AKAP10

Background: The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein interacts with both the type I and type II regulatory subunits of PKA, therefore, it is a dual-specific AKAP. This protein is highly enriched in mitochondria. It contains RGS (regulator of G protein signalling) domains, in addition to a PKA-RII subunit-binding domain. The mitochondrial localization and the presence of RGS domains may have important implications for the function of this protein in PKA and G protein signal transduction.
Alias Symbols: D-AKAP2, MGC9414, PRKA10
Protein Interaction Partner: COG3, DCTN4, MYH9, PRKAR1A, HAX1, SLC9A3R1, PDZK1, PRKAR2B, PRKAR2A,
Protein Size: 662

Molecular Weight: 71 kDa

Gene ID: 11216

NCBI Accession: NM_007202, NP_009133

UniProt: O43572

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling