DHX9 antibody (N-Term)

Catalog No. ABIN2775181

There is 1 publication for this product available. The Rabbit Polyclonal anti-DHX9 antibody is suitable to detect DHX9 in samples from Human, Mouse, Rat, Dog, Cow, Horse, Rabbit and Pig. It has been validated for WB, IHC and IP.

Quick Overview for DHX9 antibody (N-Term) (ABIN2775181)

Target: DHX9 (ATP-Dependent RNA Helicase A (DHX9))

Reactivity: Human, Mouse, Rat, Dog, Cow, Horse, Rabbit, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DHX9 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Product Details anti-DHX9 Antibody

Binding Specificity: N-Term

Purpose: DHX9 Antibody - N-terminal region

Sequence: GLHGNWTLEN AKARLNQYFQ KEKIQGEYKY TQVGPDHNRS FIAEMTIYIK

Predicted Reactivity: Cow: 100%, Dog: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against DHX9. It was validated on Western Blot and immunohistochemistry.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human DHX9

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8095207

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-DHX9 antibody (ABIN2775181)

Kawai, Amano: "BRCA1 regulates microRNA biogenesis via the DROSHA microprocessor complex." in: The Journal of cell biology, Vol. 197, Issue 2, pp. 201-8, (2012) (PubMed).

Target Details for DHX9

Target: DHX9 (ATP-Dependent RNA Helicase A (DHX9))

Alternative Name: DHX9

Background:

Background Information: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. DHX9 is a DEAD box protein with RNA helicase activity. It may participate in melting of DNA:RNA hybrids, such as those that occur during transcription, and may play a role in X-linked gene expression.DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein with RNA helicase activity. It may participate in melting of DNA:RNA hybrids, such as those that occur during transcription, and may play a role in X-linked gene expression. It contains 2 copies of a double-stranded RNA-binding domain, a DEXH core domain and an RGG box. The RNA-binding domains and RGG box influence and regulate RNA helicase activity.

Gene Name: DEAH (Asp-Glu-Ala-His) box polypeptide 9

Alternative Symbols: LKP, RHA, DDX9, NDH2, NDHII

Protein Name: ATP-dependent RNA helicase A

Molecular Weight: 141kDa

Gene ID: 1660

NCBI Accession: NP_001348

UniProt: Q08211