BAT1 antibody (C-Term)

Catalog No. ABIN2775195

This Rabbit Polyclonal antibody specifically detects BAT1 in WB. It exhibits reactivity toward Human, Rat, Dog, Mouse, Cow, Goat, Guinea Pig, Horse, Rabbit and Zebrafish (Danio rerio).It has been mentioned in 1 publication

Quick Overview for BAT1 antibody (C-Term) (ABIN2775195)

Target: BAT1 (DDX39) (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 39 (DDX39))

Reactivity: Human, Rat, Dog, Mouse, Cow, Goat, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BAT1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-BAT1 Antibody

Binding Specificity: C-Term

Purpose: BAT1 Antibody - C-terminal region

Sequence: YDMPEDSDTY LHRVARAGRF GTKGLAITFV SDENDAKILN DVQDRFEVNI

Predicted Reactivity: Cow: 100%, Dog: 100%, Goat: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against BAT1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human BAT1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8098615

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-BAT1 antibody (ABIN2775195)

Bouley, Pionneau, Varinot, Biard, Genestie, Antoine, Coulet, Stern, Stoppa-Lyonnet, Soubrier: "Proteomic analysis of BRCA1-depleted cell line reveals a putative role for replication protein A2 up-regulation in BRCA1 breast tumor development." in: Proteomics. Clinical applications, Vol. 4, Issue 5, pp. 489-98, (2010) (PubMed).

Target Details for BAT1

Target: BAT1 (DDX39) (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 39 (DDX39))

Alternative Name: BAT1

Background:

Background Information: BAT1 is a member of the DEAD protein family of ATP-dependent RNA helicases. Members of this family are involved in a number of cellular functions including initiation of translation, RNA splicing, and ribosome assembly. A cluster of genes, BAT1-BAT5, has been localized in the vicinity of the genes for TNF alpha and TNF beta. These genes are all within the human major histocompatibility complex class III region. This protein is a negative regulator of inflammation. It is also thought to be a translation initiation factor. This gene is a strong candidate gene for rheumatoid arthritis. There are multiple alternatively spliced transcript variants known for this gene but only two have been fully described. Both of these variants encode the same isoform. This gene has been found to have multiple polyadenylation sites.

Gene Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 39B

Alternative Symbols: BAT1, UAP56, D6S81E

Protein Name: Spliceosome RNA helicase DDX39B

Molecular Weight: 47kDa

Gene ID: 7919

NCBI Accession: NP_542165

UniProt: Q13838

Pathways: Ribonucleoprotein Complex Subunit Organization