DDX23 antibody (C-Term)

Catalog No. ABIN2775199

This Rabbit Polyclonal antibody specifically detects DDX23 in WB. It exhibits reactivity toward Human, Mouse, Rat, Dog, Cow, Horse, Rabbit, Guinea Pig and Zebrafish (Danio rerio).

Quick Overview for DDX23 antibody (C-Term) (ABIN2775199)

Target: DDX23 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 23 (DDX23))

Reactivity: Human, Mouse, Rat, Dog, Cow, Horse, Rabbit, Guinea Pig, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DDX23 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-DDX23 Antibody

Binding Specificity: C-Term

Sequence: EDSAVFYELK QAILESPVSS CPPELANHPD AQHKPGTILT KKRREETIFA

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against DDX23. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human DDX23

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 820 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for DDX23

Target: DDX23 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 23 (DDX23))

Alternative Name: DDX23

Background: DDX23 encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene is a component of the U5 snRNP complex, it may facilitate conformational changes in the spliceosome during nuclear pre-mRNA splicing. An alternatively spliced transcript variant has been found for this gene, but its biological validity has not been determined.
Alias Symbols: prp28, PRPF28, U5-100K, U5-100KD
Protein Interaction Partner: SUMO2, STAU1, UBC, RPA3, RPA2, RPA1, ERG, SUZ12, WBP4, PRPF4B, PAXIP1, PHF5A, SF3A3, SART1, THOC5, PURA, RNU6ATAC, RNU4ATAC, TERF2IP, TERF1, SIRT7, HNRNPA1, SF3A2, SUMO1, Prpf8, TOP1, SNW1, TP63, TP73, MEPCE, USP39, PRPF4, OTUB1, RNPS1, CD2BP2,
Protein Size: 820

Molecular Weight: 90 kDa

Gene ID: 9416

NCBI Accession: NM_004818, NP_004809

UniProt: Q9BUQ8

Pathways: Ribonucleoprotein Complex Subunit Organization