DHX8 antibody (N-Term)

Catalog No. ABIN2775203

This Rabbit Polyclonal antibody specifically detects DHX8 in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog, Rabbit, Horse and Guinea Pig.

Quick Overview for DHX8 antibody (N-Term) (ABIN2775203)

Target: DHX8 (DEAH (Asp-Glu-Ala-His) Box Polypeptide 8 (DHX8))

Reactivity: Human, Mouse, Rat, Cow, Dog, Rabbit, Horse, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DHX8 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-DHX8 Antibody

Binding Specificity: N-Term

Purpose: DHX8 Antibody - N-terminal region

Sequence: VKNGAEFTDS LISNLLRLIQ TMRPPAKPST SKDPVVKPKT EKEKLKELFP

Predicted Reactivity: Cow: 100%, Dog: 88%, Guinea Pig: 100%, Horse: 93%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against DHX8. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human DHX8

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP36380-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for DHX8

Target: DHX8 (DEAH (Asp-Glu-Ala-His) Box Polypeptide 8 (DHX8))

Alternative Name: DHX8

Background:

Background Information: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is highly homologous to yeast Prp22. This protein facilitates nuclear export of spliced mRNA by releasing the RNA from the spliceosome.

Gene Name: DEAH (Asp-Glu-Ala-His) box polypeptide 8

Alternative Symbols: DDX8, Dhr2, HRH1, PRP22, PRPF22

Protein Name: ATP-dependent RNA helicase DHX8

Molecular Weight: 139kDa

Gene ID: 1659

NCBI Accession: NP_004932

UniProt: Q14562