DDX42 antibody (N-Term)

Catalog No. ABIN2775212

The Rabbit Polyclonal anti-DDX42 antibody (ABIN2775212) specifically detects DDX42 in WB. The antibody is reactive with Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse and Rabbit samples.

Quick Overview for DDX42 antibody (N-Term) (ABIN2775212)

Target: DDX42 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 42 (DDX42))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DDX42 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-DDX42 Antibody

Binding Specificity: N-Term

Purpose: DDX42 Antibody - N-terminal region

Sequence: PLEAFMAEVE DQAARDMKRL EEKDKERKNV KGIRDDIEEE DDQEAYFRYM

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against DDX42. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human DDX42

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8098620

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for DDX42

Target: DDX42 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 42 (DDX42))

Alternative Name: DDX42

Background:

Background Information: DDX42 is a member of the Asp-Glu-Ala-Asp (DEAD) box protein family. Members of this protein family are putative RNA helicases, and are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. DDX42 is a ATP-dependent RNA helicase. DDX42 binds to partially double-stranded RNAs (dsRNAs) in order to unwind RNA secondary structures. It also mediates RNA duplex formation thereby displacing the single-strand RNA binding protein.This gene encodes a member of the Asp-Glu-Ala-Asp (DEAD) box protein family. Members of this protein family are putative RNA helicases, and are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. Two transcript variants encoding the same protein have been identified for this gene.

Gene Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 42

Alternative Symbols: RHELP, RNAHP, SF3B8, DDX42P, SF3b125

Protein Name: ATP-dependent RNA helicase DDX42

Molecular Weight: 103kDa

Gene ID: 11325

NCBI Accession: NP_031398

UniProt: Q86XP3