EIF4A3 antibody (Middle Region)

Catalog No. ABIN2775226

The Rabbit Polyclonal anti-EIF4A3 antibody is suitable to detect EIF4A3 in samples from Human, Mouse, Rat, Dog, Zebrafish (Danio rerio), Cow, Guinea Pig, Horse and Saccharomyces cerevisiae. It has been validated for WB.

Quick Overview for EIF4A3 antibody (Middle Region) (ABIN2775226)

Target: EIF4A3 (Eukaryotic Translation Initiation Factor 4A3 (EIF4A3))

Reactivity: Human, Mouse, Rat, Dog, Zebrafish (Danio rerio), Cow, Guinea Pig, Horse, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF4A3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-EIF4A3 Antibody

Binding Specificity: Middle Region

Purpose: DDX48 Antibody - middle region

Sequence: QCHACIGGTN VGEDIRKLDY GQHVVAGTPG RVFDMIRRRS LRTRAIKMLV

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rat: 100%, Yeast: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against DDX48. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human DDX48

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8099974

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for EIF4A3

Target: EIF4A3 (Eukaryotic Translation Initiation Factor 4A3 (EIF4A3))

Alternative Name: DDX48

Background:

Background Information: DDX48 encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene is a nuclear matrix protein. Its amino acid sequence is highly similar to the amino acid sequences of the translation initiation factors eIF4AI and eIF4AII, two other members of the DEAD box protein family.

Gene Name: Eukaryotic translation initiation factor 4A3

Alternative Symbols: Fal1, RCPS, DDX48, MUK34, NUK34, NMP265, eIF4AIII, eIF4A-III, eIF-4A-III

Protein Name: Eukaryotic initiation factor 4A-III

Molecular Weight: 45kDa

Gene ID: 9775

NCBI Accession: NP_055555

UniProt: P38919