DDX47 antibody (N-Term)

Catalog No. ABIN2775232

This Rabbit Polyclonal antibody specifically detects DDX47 in WB. It exhibits reactivity toward Human, Mouse, Rat, Dog, Rabbit, Horse, Guinea Pig and Pigand has been mentioned in 1 publication.

Quick Overview for DDX47 antibody (N-Term) (ABIN2775232)

Target: DDX47 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 47 (DDX47))

Reactivity: Human, Mouse, Rat, Dog, Rabbit, Horse, Guinea Pig, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DDX47 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-DDX47 Antibody

Binding Specificity: N-Term

Sequence: AAPEEHDSPT EASQPIVEEE ETKTFKDLGV TDVLCEACDQ LGWTKPTKIQ

Predicted Reactivity: Dog: 85%, Guinea Pig: 83%, Horse: 92%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against DDX47. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human DDX47

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 455 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-DDX47 antibody (ABIN2775232)

Mehrle, Rosenfelder, Schupp, del Val, Arlt, Hahne, Bechtel, Simpson, Hofmann, Hide, Glatting, Huber, Pepperkok, Poustka, Wiemann: "The LIFEdb database in 2006." in: Nucleic acids research, Vol. 34, Issue Database issue, pp. D415-8, (2005) (PubMed).

Target Details for DDX47

Target: DDX47 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 47 (DDX47))

Alternative Name: DDX47

Background: DDX47 is a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. DDX47 can shuttle between the nucleus and the cytoplasm, and has an RNA-independent ATPase activity. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene can shuttle between the nucleus and the cytoplasm, and has an RNA-independent ATPase activity. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Alias Symbols: DKFZp564O176, E4-DBP, FLJ30012, HQ0256, MSTP162, RRP3
Protein Interaction Partner: UBC, EED, rev, PARK2, SRPK1, MLH1, KRAS, FN1, APP, EXOSC4, CAND1, PRNP, SUMO1, HDGF, SUMO2, HDAC5, SART3, Rrp1b,
Protein Size: 455

Molecular Weight: 50 kDa

Gene ID: 51202

NCBI Accession: NM_016355, NP_057439

UniProt: Q9H0S4