DDX28 antibody (N-Term)

Catalog No. ABIN2775241

The Rabbit Polyclonal anti-DDX28 antibody is suitable to detect DDX28 in samples from Human, Mouse, Rat, Cow and Pig. It has been validated for WB.

Quick Overview for DDX28 antibody (N-Term) (ABIN2775241)

Target: DDX28 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 28 (DDX28))

Reactivity: Human, Mouse, Rat, Cow, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DDX28 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-DDX28 Antibody

Binding Specificity: N-Term

Purpose: DDX28 Antibody - N-terminal region

Sequence: FSIERAQQEA PAVRKLSSKG SFADLGLEPR VLHALQEAAP EVVQPTTVQS

Predicted Reactivity: Cow: 79%, Human: 100%, Mouse: 83%, Pig: 85%, Rat: 83%

Characteristics: This is a rabbit polyclonal antibody against DDX28. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human DDX28

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP36457-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for DDX28

Target: DDX28 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 28 (DDX28))

Alternative Name: DDX28

Background:

Background Information: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. DDX28 is an RNA-dependent ATPase. DDX28 is localized in the mitochondria and the nucleus, and can be transported between the mitochondria and the nucleus.DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene is intronless. It encodes an RNA-dependent ATPase. The encoded protein is localized in the mitochondria and the nucleus, and can be transported between the mitochondria and the nucleus.

Gene Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 28

Alternative Symbols: MDDX28

Protein Name: Probable ATP-dependent RNA helicase DDX28

Molecular Weight: 59kDa

Gene ID: 55794

NCBI Accession: NP_060850

UniProt: Q9NUL7