DDX31 antibody (N-Term)

Catalog No. ABIN2775254

The Rabbit Polyclonal anti-DDX31 antibody is suitable to detect DDX31 in samples from Human, Mouse, Rat and Guinea Pig. It has been validated for WB.

Quick Overview for DDX31 antibody (N-Term) (ABIN2775254)

Target: DDX31 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 31 (DDX31))

Reactivity: Human, Mouse, Rat, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DDX31 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-DDX31 Antibody

Binding Specificity: N-Term

Purpose: DDX31 Antibody - N-terminal region

Sequence: QASSEAPPAK RRNETSFLPA KKTSVKETQR TFKGNAQKMF SPKKHSVSTS

Predicted Reactivity: Guinea Pig: 83%, Human: 100%, Mouse: 86%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against DDX31. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human DDX31

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8098614

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for DDX31

Target: DDX31 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 31 (DDX31))

Alternative Name: DDX31

Background:

Background Information: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. DDX31 is a member of this family. The function of this member has not been determined. Alternative splicing of this gene generates 2 transcript variants.

Gene Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 31

Alternative Symbols: PPP1R25

Protein Name: Probable ATP-dependent RNA helicase DDX31

Molecular Weight: 64kDa

Gene ID: 64794

NCBI Accession: NP_619526

UniProt: Q9H8H2