DDX54 antibody (C-Term)

Catalog No. ABIN2775258

The Rabbit Polyclonal anti-DDX54 antibody has been validated for WB. It is suitable to detect DDX54 in samples from Human, Dog, Cow, Horse, Mouse, Rat, Guinea Pig, Rabbit and Zebrafish (Danio rerio).

Quick Overview for DDX54 antibody (C-Term) (ABIN2775258)

Target: DDX54 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 54 (DDX54))

Reactivity: Human, Dog, Cow, Horse, Mouse, Rat, Guinea Pig, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DDX54 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-DDX54 Antibody

Binding Specificity: C-Term

Sequence: GPNRGAKRRR EEARQRDQEF YIPYRPKDFD SERGLSISGE GGAFEQQAAG

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 85%, Horse: 93%, Human: 100%, Mouse: 93%, Rabbit: 92%, Rat: 93%, Zebrafish: 92%

Characteristics: This is a rabbit polyclonal antibody against DDX54. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human DDX54

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 881 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for DDX54

Target: DDX54 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 54 (DDX54))

Alternative Name: DDX54

Background: DDX54 is a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein interacts in a hormone-dependent manner with nuclear receptors and represses their transcriptional activity.This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene interacts in a hormone-dependent manner with nuclear receptors and represses their transcriptional activity.
Alias Symbols: DP97, MGC2835
Protein Interaction Partner: VCP, UBC, SRPK3, HIPK1, SEMA4A, TMED9, WHSC1, POLR2G, CSNK2A1, ESR1, APP, SIRT7, Nedd4, NEDD4L, PGR, ESR2, NR3C1, OTUB1,
Protein Size: 881

Molecular Weight: 97 kDa

Gene ID: 79039

NCBI Accession: NM_024072, NP_076977

UniProt: Q8TDD1

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway