DDX54 antibody (AA 570-650)

Catalog No. ABIN7214542

This Rabbit Polyclonal antibody specifically detects DDX54 in WB and ELISA. It exhibits reactivity toward Human and Mouse.

Quick Overview for DDX54 antibody (AA 570-650) (ABIN7214542)

Target: DDX54 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 54 (DDX54))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DDX54 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-DDX54 Antibody

Binding Specificity: AA 570-650

Purpose: Rabbit Anti-DDX54 Polyclonal Antibody

Specificity: DDX54 Polyclonal Antibody detects endogenous levels of DDX54 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human DDX54 at AA range: 570-650

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,ELISA 1:10000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for DDX54

Target: DDX54 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 54 (DDX54))

Alternative Name: DDX54

Background: DDX54, ATP-dependent RNA helicase DDX54, ATP-dependent RNA helicase DP97, DEAD box RNA helicase 97 kDa, DEAD box protein 54DDX54 encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The nucleolar protein (DEAD-box helicase 54) encoded by DDX54 interacts in a hormone-dependent manner with nuclear receptors, and represses their transcriptional activity. Alternative splice variants that encode different isoforms have been found for DDX54.

Gene ID: 79039

UniProt: Q8TDD1

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway