DHX30 antibody (N-Term)

Catalog No. ABIN2775264

Product Details anti-DHX30 Antibody

Target: DHX30 (DEAH (Asp-Glu-Ala-His) Box Polypeptide 30 (DHX30))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Cow, Dog, Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DHX30 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: AESGMAPGGP GEGDGSLVNA SRDLLKEFPQ PKNLLNSVIG RALGISHAKD

Predicted Reactivity: Cow: 93%, Dog: 93%, Human: 100%, Mouse: 93%, Pig: 93%, Rabbit: 93%

Characteristics: This is a rabbit polyclonal antibody against DHX30. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human DHX30

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 361 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for DHX30

Target: DHX30 (DEAH (Asp-Glu-Ala-His) Box Polypeptide 30 (DHX30))

Alternative Name: DHX30 (DHX30 Products)

Background: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. DHX30 is a member of this family.DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a member of this family. The encoded protein has 97 % sequence identity with the mouse HELG protein. Alternative splicing of this gene generates 3 transcript variants.
Alias Symbols: DDX30, FLJ11214, KIAA0890, RETCOR
Protein Interaction Partner: SUMO2, SUMO3, IVNS1ABP, STAU1, GRSF1, UBC, SUMO1, NEDD8, Fbxl16, WWOX, SUZ12, RNF2, EZH2, NEDD4, TARDBP, HNRNPD, EIF2AK2, SKIL, FN1, ESR1, NDUFA2, ILF3, GNAI2, RAB31, GRK5, CAND1, COPS5, CUL1, CUL3, CUL4B, CUL5, SIRT7, HNRNPA1, HDGF, DHX58, OAS3, Sgol2, Y
Protein Size: 361

Molecular Weight: 40 kDa

Gene ID: 22907

NCBI Accession: NM_138614, NP_619519

Pathways: Chromatin Binding