BTRC antibody (C-Term)

Catalog No. ABIN2775273

The Rabbit Polyclonal anti-BTRC antibody has been validated for WB. It is suitable to detect BTRC in samples from Human, Mouse, Rat, Cow, Dog, Guinea Pig, Rabbit and Horse.

Quick Overview for BTRC antibody (C-Term) (ABIN2775273)

Target: BTRC (beta-Transducin Repeat Containing (BTRC))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Rabbit, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BTRC antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-BTRC Antibody

Binding Specificity: C-Term

Sequence: LVEHSGRVFR LQFDEFQIVS SSHDDTILIW DFLNDPAAHA EPPRSPSRTY

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 93%, Human: 100%, Mouse: 93%, Rabbit: 100%, Rat: 86%

Characteristics: This is a rabbit polyclonal antibody against Btrc. It was validated on Western Blot.

Purification: Affinity Purified

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 605 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for BTRC

Target: BTRC (beta-Transducin Repeat Containing (BTRC))

Alternative Name: Btrc

Background: Btrc is a substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. It recognizes and binds to phosphorylated target proteins. SCF(BTRC) mediates the ubiquitination of CTNNB1 and participates in Wnt signaling. SCF(BTRC) mediates the ubiquitination of NFKBIA, NFKBIB and NFKBIE, the degradation frees the associated NFKB1 to translocate into the nucleus and to activate transcription. Ubiquitination of NFKBIA occurs at 'Lys-21' and 'Lys-22'. SCF(BTRC) mediates the ubiquitination of phosphorylated NFKB1/nuclear factor NF-kappa-B p105 subunit, ATF4, SMAD3, SMAD4, CDC25A, DLG1, FBXO5 and probably NFKB2. SCF(BTRC) mediates the ubiquitination of phosphorylated SNAI1.
Alias Symbols: Beta-Trcp1, FWD1, Fbw1a, KIAA4123, Slimb, b-TrCP, beta-TrCP, mKIAA4123, HOS
Protein Interaction Partner: ZRANB1, Tpx2, Taz, Per2, Cul1, Skp1a, Reg1, Stat1, Nfe2l2, Per1, Htt, Cops2, Ikbkb, Nkx3-2, Cdc25b, Nfkbia, Gli2,
Protein Size: 605

Molecular Weight: 69 kDa

Gene ID: 12234

NCBI Accession: NM_001037758, NP_001032847

UniProt: Q3ULA2

Pathways: Cell Division Cycle, Hedgehog Signaling