CD8B antibody (N-Term)

Catalog No. ABIN2775290

This Rabbit Polyclonal antibody specifically detects CD8B in WB. It exhibits reactivity toward Human, Rat, Dog, Guinea Pig and Horse.

Quick Overview for CD8B antibody (N-Term) (ABIN2775290)

Target: CD8B (CD8b Molecule (CD8B))

Reactivity: Human, Rat, Dog, Guinea Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD8B antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CD8B Antibody

Binding Specificity: N-Term

Sequence: RIYWLRQRQA PSSDSHHEFL ALWDSAKGTI HGEEVEQEKI AVFRDASRFI

Predicted Reactivity: Dog: 93%, Guinea Pig: 86%, Horse: 86%, Human: 100%, Rat: 86%

Characteristics: This is a rabbit polyclonal antibody against CD8B. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human CD8B

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 246 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for CD8B

Target: CD8B (CD8b Molecule (CD8B))

Alternative Name: CD8B

Background: The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. CD8B is the CD8 beta chain isoforms.The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 beta chain isoforms. Multiple alternatively spliced transcript variants encoding distinct membrane associated or secreted isoforms have been described. A pseudogene, also located on chromosome 2, has been identified.
Alias Symbols: CD8B, LYT3, Leu2, Ly3, LY3, P37, LEU2, CD8B1
Protein Interaction Partner: ELAVL1, CD8A, CD3D, CD8B, ST3GAL4, LCK,
Protein Size: 246

Molecular Weight: 27 kDa

Gene ID: 926

NCBI Accession: NM_172099, NP_742097

UniProt: Q53QL8

Pathways: TCR Signaling