EBI3 antibody (Middle Region)

Catalog No. ABIN2775323

The Rabbit Polyclonal anti-EBI3 antibody (ABIN2775323) specifically detects EBI3 in WB. The antibody is reactive with Human, Dog and Guinea Pig samples.

Quick Overview for EBI3 antibody (Middle Region) (ABIN2775323)

Target: EBI3 (IL-27b) (Interleukin-27 subunit beta (IL-27b))

Reactivity: Human, Dog, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EBI3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-EBI3 Antibody

Binding Specificity: Middle Region

Purpose: EBI3 Antibody - middle region

Sequence: TAVHPWGSSS SFVPFITEHI IKPDPPEGVR LSPLAERQLQ VQWEPPGSWP

Predicted Reactivity: Dog: 91%, Guinea Pig: 93%, Human: 100%

Characteristics: This is a rabbit polyclonal antibody against EBI3. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human EBI3

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP42257-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for EBI3

Target: EBI3 (IL-27b) (Interleukin-27 subunit beta (IL-27b))

Alternative Name: EBI3

Background:

Targeted Subunit: beta

Background Information: This gene was identified by its induced expression in B lymphocytes in response Epstein-Barr virus infection. It encodes a secreted glycoprotein belonging to the hematopoietin receptor family, and heterodimerizes with a 28 kDa protein to form interleukin 27 (IL-27). IL-27 regulates T cell and inflammatory responses, in part by activating the Jak/STAT pathway of CD4+ T cells.

Gene Name: Epstein-Barr virus induced 3

Alternative Symbols: IL27B, IL35B, IL-27B

Protein Name: Interleukin-27 subunit beta

Molecular Weight: 23kDa

Gene ID: 10148

NCBI Accession: NP_005746

UniProt: Q14213