MYH1 antibody (N-Term)

Catalog No. ABIN2775330

There are 2+ publications for this product available. The Rabbit Polyclonal anti-MYH1 antibody is suitable to detect MYH1 in samples from Human, Mouse, Rat, Rabbit, Cow, Horse, Sheep, Dog, Guinea Pig and Zebrafish (Danio rerio). It has been validated for WB.

Quick Overview for MYH1 antibody (N-Term) (ABIN2775330)

Target: MYH1 (Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1))

Reactivity: Human, Mouse, Rat, Rabbit, Cow, Horse, Sheep, Dog, Guinea Pig, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MYH1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MYH1 Antibody

Binding Specificity: N-Term

Purpose: MYH1 Antibody - N-terminal region

Sequence: KTSVFVVDPK ESFVKATVQS REGGKVTAKT EAGATVTVKD DQVFPMNPPK

Predicted Reactivity: Cow: 100%, Dog: 93%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Sheep: 100%, Zebrafish: 85%

Characteristics: This is a rabbit polyclonal antibody against MYH1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human MYH1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP42269-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-MYH1 antibody (ABIN2775330)

Rouillon, Poupiot, Zocevic, Amor, Léger, Garcia, Camadro, Wong, Pinilla, Cosette, Coenen-Stass, Mcclorey, Roberts, Wood, Servais, Udd, Voit, Richard, Svinartchouk: "Serum proteomic profiling reveals fragments of MYOM3 as potential biomarkers for monitoring the outcome of therapeutic interventions in muscular dystrophies." in: Human molecular genetics, Vol. 24, Issue 17, pp. 4916-32, (2016) (PubMed).

Avezzù, Agostini, Pucciarelli, Lise, Urso, Mammi, Maretto, Enzo, Pastrello, Lise, Nitti, Viel: "The role of MYH gene in genetic predisposition to colorectal cancer: another piece of the puzzle." in: Cancer letters, Vol. 268, Issue 2, pp. 308-13, (2008) (PubMed).

Target Details for MYH1

Target: MYH1 (Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1))

Alternative Name: MYH1

Background:

Background Information: Myosin is a major contractile protein which converts chemical energy into mechanical energy through the hydrolysis of ATP. Myosin is a hexameric protein composed of a pair of myosin heavy chains (MYH) and two pairs of nonidentical light chains. Myosin hea

Gene Name: Myosin, heavy chain 1, skeletal muscle, adult

Alternative Symbols: MYHa, HEL71, MYHSA1, MyHC-2x, MyHC-2X/D

Protein Name: Myosin-1

Molecular Weight: 223 kDa

Gene ID: 4619

NCBI Accession: NP_005954

UniProt: P12882