Cytokeratin 1 antibody (Middle Region)

Catalog No. ABIN2775333

The Rabbit Polyclonal anti-Cytokeratin 1 antibody is suitable to detect Cytokeratin 1 in samples from Human, Rat, Rabbit and Dog. It has been validated for WB and IHC.

Quick Overview for Cytokeratin 1 antibody (Middle Region) (ABIN2775333)

Target: Cytokeratin 1 (KRT1) (Keratin 1 (KRT1))

Reactivity: Human, Rat, Rabbit, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cytokeratin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Cytokeratin 1 Antibody

Binding Specificity: Middle Region

Purpose: KRT1 Antibody - middle region

Sequence: GSYGSGSSSG GYRGGSGGGG GGSSGGRGSG GGSSGGSIGG RGSSSGGVKS

Predicted Reactivity: Dog: 86%, Human: 100%, Rabbit: 93%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against KRT1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human KRT1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP42278-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for Cytokeratin 1

Target: Cytokeratin 1 (KRT1) (Keratin 1 (KRT1))

Alternative Name: KRT1

Background:

Background Information: KRT1 is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the spinous and granular layers of the epidermis with family member KRT10 and mutations in these genes have been associated with bullous congenital ichthyosiform erythroderma.The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the spinous and granular layers of the epidermis with family member KRT10 and mutations in these genes have been associated with bullous congenital ichthyosiform erythroderma. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Gene Name: Keratin 1

Alternative Symbols: K1, CK1, EHK, EHK1, EPPK, KRT1A, NEPPK

Protein Name: Keratin, type II cytoskeletal 1

Molecular Weight: 66kDa

Gene ID: 3848

NCBI Accession: NP_006112

UniProt: P04264