EIF2AK1 antibody (N-Term)

Catalog No. ABIN2775397

This anti-EIF2AK1 antibody is a Rabbit Polyclonal antibody detecting EIF2AK1 in WB and IHC. Suitable for Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit and Zebrafish (Danio rerio).

Quick Overview for EIF2AK1 antibody (N-Term) (ABIN2775397)

Target: EIF2AK1 (Eukaryotic Translation Initiation Factor 2-alpha Kinase 1 (EIF2AK1))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF2AK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-EIF2AK1 Antibody

Binding Specificity: N-Term

Sequence: TCSDEFSSLR LHHNRAITHL MRSAKERVRQ DPCEDISRIQ KIRSREVALE

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 79%

Characteristics: This is a rabbit polyclonal antibody against EIF2AK1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human EIF2AK1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 630 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for EIF2AK1

Target: EIF2AK1 (Eukaryotic Translation Initiation Factor 2-alpha Kinase 1 (EIF2AK1))

Alternative Name: EIF2AK1

Background: EIF2AK1 acts at the level of translation initiation to downregulate protein synthesis in response to stress. The protein is a kinase that can be inactivated by hemin. Two transcript variants encoding different isoforms have been found for this gene.The HRI gene is localized to 7p22 where its 3' end slightly overlaps the 3' end of the gene JTV1. The two genes are transcribed from opposite strands. Studies in rat and rabbit suggest that the HRI gene product phosphorylates the alpha subunit of eukaryotic initiation factor 2. Its kinase activity is induced by low levels of heme and inhibited by the presence of heme. Sequence Note: The sequence AF181071.1 is a chimeric mRNA clone. Only the heme-regulated initiation factor 2-alpha kinase region was propagated into this RefSeq record.
Alias Symbols: HRI, KIAA1369, HCR
Protein Interaction Partner: MDM2, UBC, HSP90AA1, LIG4, CDC37, EIF2AK1, EIF2S1,
Protein Size: 630

Molecular Weight: 71 kDa

Gene ID: 27102

NCBI Accession: NM_014413, NP_055228

UniProt: Q9BQI3

Pathways: Hepatitis C