CHIA antibody (N-Term)

Catalog No. ABIN2775494

Cited in 2+ publications. The Rabbit Polyclonal anti-CHIA antibody (ABIN2775494) specifically detects CHIA in WB. The antibody is reactive with Human, Mouse, Rat, Cow, Dog, Horse, Pig, Zebrafish (Danio rerio) and Guinea Pig samples.

Quick Overview for CHIA antibody (N-Term) (ABIN2775494)

Target: CHIA (Chitinase, Acidic (CHIA))

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Pig, Zebrafish (Danio rerio), Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHIA antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CHIA Antibody

Binding Specificity: N-Term

Purpose: CHIA Antibody - N-terminal region

Sequence: MVSTPENRQT FITSVIKFLR QYEFDGLDFD WEYPGSRGSP PQDKHLFTVL

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 79%, Horse: 86%, Human: 100%, Mouse: 100%, Pig: 93%, Rat: 100%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against CHIA. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human CHIA

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP42601-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-CHIA antibody (ABIN2775494)

Strobel, Roswag, Becker, Trenczek, Encarnação: "Insectivorous bats digest chitin in the stomach using acidic mammalian chitinase." in: PLoS ONE, Vol. 8, Issue 9, pp. e72770, (2013) (PubMed).

Fukushima, Nashida, Haga-Tsujimura, Mataga: "Chitinase expression in parotid glands of non-obese diabetic mice." in: Oral diseases, Vol. 18, Issue 5, pp. 506-12, (2012) (PubMed).

Target Details for CHIA

Target: CHIA (Chitinase, Acidic (CHIA))

Alternative Name: CHIA

Background:

Background Information: CHIA belongs to the glycosyl hydrolase 18 family. Chitinase class II subfamily. It contains 1 chitin-binding type-2 domain. The protein degrades chitin and chitotriose. And it may participate in the defense against nematodes and other pathogens.

Gene Name: Chitinase, acidic

Alternative Symbols: CHIT2, AMCASE, TSA1902

Protein Name: Acidic mammalian chitinase

Molecular Weight: 40kDa

Gene ID: 27159

NCBI Accession: NP_068569

UniProt: Q9BZP6