Chitinase, Acidic (CHIA) (N-Term) antibody

Details for Product No. ABIN2775494
Antigen
Epitope
N-Term
11
9
7
2
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus), Zebrafish (Danio rerio)
55
26
15
3
2
2
2
1
1
Host
Rabbit
52
3
Clonality
Polyclonal
Conjugate
Un-conjugated
3
2
2
2
2
2
1
1
1
1
1
1
Application
Western Blotting (WB)
50
22
17
14
3
1
1
Options
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human CHIA
Blocking Peptide Blocking peptide for this product available: ABIN974917
Sequence MVSTPENRQT FITSVIKFLR QYEFDGLDFD WEYPGSRGSP PQDKHLFTVL
Predicted Reactivity Cow: 93%, Dog: 100%, Guinea Pig: 79%, Horse: 86%, Human: 100%, Mouse: 100%, Pig: 93%, Rat: 100%, Zebrafish: 86%
Characteristics This is a rabbit polyclonal antibody against CHIA. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Antigen
Alternative Name CHIA (CHIA Antibody Abstract)
Background CHIA belongs to the glycosyl hydrolase 18 family. Chitinase class II subfamily. It contains 1 chitin-binding type-2 domain. The protein degrades chitin and chitotriose. And it may participate in the defense against nematodes and other pathogens.
Alias Symbols: 2200003E03Rik, AMCase, CHIT2, DKFZp313J1722, RP5-1125M8.1, TSA1902, AMCASE
Protein Size: 368
Molecular Weight 40 kDa
Gene ID 27159
NCBI Accession NM_021797, NP_068569
UniProt Q9BZP6
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 368 AA

Restrictions For Research Use only
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Supplier Images
Image no. 1 for anti-Chitinase, Acidic (CHIA) (N-Term) antibody (ABIN2775494) WB Suggested Anti-CHIA Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:62500 Posit...
Product cited in: Strobel, Roswag, Becker, Trenczek, Encarnação: "Insectivorous bats digest chitin in the stomach using acidic mammalian chitinase." in: PLoS ONE, Vol. 8, Issue 9, pp. e72770, 2013 (PubMed).

Fukushima, Nashida, Haga-Tsujimura, Mataga: "Chitinase expression in parotid glands of non-obese diabetic mice." in: Oral diseases, Vol. 18, Issue 5, pp. 506-12, 2012 (PubMed).