HDAC9 antibody (Middle Region)

Catalog No. ABIN2775579

The Rabbit Polyclonal anti-HDAC9 antibody has been validated for WB. It is suitable to detect HDAC9 in samples from Human, Rat, Mouse, Dog, Guinea Pig, Sheep, Cow, Horse, Rabbit and Zebrafish (Danio rerio). There is 1 publication available.

Quick Overview for HDAC9 antibody (Middle Region) (ABIN2775579)

Target: HDAC9 (Histone Deacetylase 9 (HDAC9))

Reactivity: Human, Rat, Mouse, Dog, Guinea Pig, Sheep, Cow, Horse, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HDAC9 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-HDAC9 Antibody

Binding Specificity: Middle Region

Sequence: GQVGAVKVKE EPVDSDEDAQ IQEMESGEQA AFMQQVIGKD LAPGFVIKVI

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 93%, Rat: 100%, Sheep: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against HDAC9. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human HDAC9

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 590 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-HDAC9 antibody (ABIN2775579)

Su, Becker, Kozyrskyj, Hayglass: "Epigenetic regulation of established human type 1 versus type 2 cytokine responses." in: The Journal of allergy and clinical immunology, Vol. 121, Issue 1, pp. 57-63.e3, (2008) (PubMed).

Target Details for HDAC9

Target: HDAC9 (Histone Deacetylase 9 (HDAC9))

Alternative Name: HDAC9

Background: Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. HDAC9 has sequence homology to members of the histone deacetylase family. The MITR protein lacks the histone deacetylase catalytic domain. It represses MEF2 activity through recruitment of multicomponent corepressor complexes that include CtBP and HDACs. HDAC9 may play a role in hematopoiesis. Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene has sequence homology to members of the histone deacetylase family. This gene is orthologous to the Xenopus and mouse MITR genes. The MITR protein lacks the histone deacetylase catalytic domain. It represses MEF2 activity through recruitment of multicomponent corepressor complexes that include CtBP and HDACs. This encoded protein may play a role in hematopoiesis. Multiple alternatively spliced transcripts have been described for this gene but the full-length nature of some of them has not been determined.
Alias Symbols: DKFZp779K1053, HD7, HDAC, HDAC7, HDAC7B, HDAC9B, HDAC9FL, HDRP, KIAA0744, MITR, HD9, HD7b
Protein Interaction Partner: NOP2, MKI67, EIF6, RCC1, ADAR, TOP1, TMPO, FIP1L1, CCAR2, TPX2, RBM14, ZC3H11A, HDAC9, NUP93, SMC3, H1FX, TPR, HINT1, CTBP1, BCL6, Zfp521, TRIM29, TP53, MAML1, SMARCA4, PARP1, CEBPB, H3F3A, RL2, CAMK1, YWHAE, HDAC1, SFN, MEF2B, ANKRA2, RFXANK, PKD1, SYK,
Protein Size: 590

Molecular Weight: 66 kDa

Gene ID: 9734

NCBI Accession: NM_014707, NP_055522

UniProt: A7E2F3

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development