Axin antibody (N-Term)

Catalog No. ABIN2775612

This Rabbit Polyclonal antibody specifically detects Axin in WB. It exhibits reactivity toward Human and Mouse.

Quick Overview for Axin antibody (N-Term) (ABIN2775612)

Target: Axin (AXIN1) (Axin 1 (AXIN1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Axin antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Axin Antibody

Binding Specificity: N-Term

Sequence: TVGRDQALGA RQAERPWPPH SHPSTPEPSV RNDGKRRFMG VRRQGSRGAG

Characteristics: This is a rabbit polyclonal antibody against Axin1. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N-terminal region of Mouse Axin1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 993 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for Axin

Target: Axin (AXIN1) (Axin 1 (AXIN1))

Alternative Name: Axin1

Background: Axin1 is a component of the beta-catenin destruction complex required for regulating CTNNB1 levels through phosphorylation and ubiquitination, and modulating Wnt-signaling. Axin1 controls dorsoventral patterning via two opposing effects, down-regulates beta-catenin to inhibit the Wnt signaling pathway and ventralize embryos, but also dorsalizes embryos by activating a Wnt-independent JNK signaling pathway. It also facilitates the phosphorylation of APC by GSK3B, facilitates the phosphorylation of TP53 by HIPK2 upon ultraviolet irradiation and enhances TGF-beta signaling by recruiting the RNF111 E3 ubiquitin ligase and promoting the degradation of inhibitory SMAD7.
Alias Symbols: AI316800, Axin, Fu, Kb, Ki, fused, kinky, knobbly
Protein Interaction Partner: Lrp5, Gsk3b, Prmt1, Ctnnb1, GSK3A, Trp53, Hipk2, Nck2, Lrrk2, ITCH, WWP1, MAP3K1, Kif3a, Cops2, Dvl2, Axin1, Apc, Dixdc1, Ppp2r1a, Map3k4, Ppp2ca, Rnf111, Rnf11, Smad7, Smad6, Smad3, Dvl1, Cdh2, APC2,
Protein Size: 993

Molecular Weight: 110 kDa

Gene ID: 12005

NCBI Accession: XM_914907, XP_920000

UniProt: O70239

Pathways: WNT Signaling, Sensory Perception of Sound, Regulation of G-Protein Coupled Receptor Protein Signaling