PKC zeta antibody (N-Term)

Catalog No. ABIN2775614

This anti-PKC zeta antibody is a Rabbit Polyclonal antibody detecting PKC zeta in WB. Suitable for Human, Mouse, Rat, Rabbit, Cow, Dog, Horse and Guinea Pig.

Quick Overview for PKC zeta antibody (N-Term) (ABIN2775614)

Target: PKC zeta (PRKCZ) (Protein Kinase C, zeta (PRKCZ))

Reactivity: Human, Mouse, Rat, Rabbit, Cow, Dog, Horse, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKC zeta antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PKC zeta Antibody

Binding Specificity: N-Term

Sequence: MDSVMPSQEP PVDDKNEDAD LPSEETDGIA YISSSRKHDS IKDDSEDLKP

Predicted Reactivity: Cow: 77%, Dog: 75%, Guinea Pig: 75%, Horse: 75%, Human: 100%, Mouse: 92%, Rabbit: 79%, Rat: 92%

Characteristics: This is a rabbit polyclonal antibody against PRKCZ. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human PRKCZ

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 409 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for PKC zeta

Target: PKC zeta (PRKCZ) (Protein Kinase C, zeta (PRKCZ))

Alternative Name: PRKCZ

Background: Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes, it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC.Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes, it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC. Alternative splicing results in multiple transcript variants encoding different isoforms.
Alias Symbols: PKC2, PKC-ZETA
Protein Interaction Partner: MAPK7, PRKCZ, AKT2, AKT1, Sqstm1, PARD6A, NUMB, NPM1, HIST1H1B, CFL1, C1QBP, HSP90AA1, NCF1, MARCKS, PPP1R14A, HIST1H1A, UBC, VHL, LRRK2, AJUBA, PARD6B, PAWR, Smurf1, Smurf2, Pard3, HIST3H3, CD44, NCOA3, TFF1, RELA, ESR1, MBP, TRIM41, PIAS4, CCDC115, ZNF7
Protein Size: 409

Molecular Weight: 45 kDa

Gene ID: 5590

NCBI Accession: NM_001033581, NP_001028753

UniProt: Q05513

Pathways: NF-kappaB Signaling, RTK Signaling, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Synaptic Membrane, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling