PARD3 antibody (Middle Region)

Catalog No. ABIN2775627

The Rabbit Polyclonal anti-PARD3 antibody (ABIN2775627) specifically detects PARD3 in WB and IHC. The antibody is reactive with Human and Guinea Pig samples.

Quick Overview for PARD3 antibody (Middle Region) (ABIN2775627)

Target: PARD3 (Par-3 Partitioning Defective 3 Homolog (PARD3))

Reactivity: Human, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PARD3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-PARD3 Antibody

Binding Specificity: Middle Region

Purpose: PARD3 Antibody - middle region

Sequence: EQQMKKQPPS EGPSNYDSYK KVQDPSYAPP KGPFRQDVPP SPSQVARLNR

Predicted Reactivity: Guinea Pig: 79%, Human: 100%

Characteristics: This is a rabbit polyclonal antibody against PARD3. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human PARD3

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP42910-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for PARD3

Target: PARD3 (Par-3 Partitioning Defective 3 Homolog (PARD3))

Alternative Name: PARD3

Background:

Background Information: PARD proteins, which were first identified in C. elegans, are essential for asymmetric cell division and polarized growth, whereas CDC42 (MIM 116952) mediates the establishment of cell polarity. The CDC42 GTPase, which is controlled by nucleotide exchange

Gene Name: Par-3 partitioning defective 3 homolog (C. elegans)

Alternative Symbols: Baz, ASIP, PAR3, PARD-3, PARD3A, SE2-5T2, PPP1R118, SE2-5L16, SE2-5LT1, PAR3alpha

Protein Name: Partitioning defective 3 homolog

Molecular Weight: 151kDa

Gene ID: 56288

NCBI Accession: NP_062565

UniProt: Q8TEW0

Pathways: Cell-Cell Junction Organization