HERC4 antibody (N-Term)

Catalog No. ABIN2775668

The Rabbit Polyclonal anti-HERC4 antibody is suitable to detect HERC4 in samples from Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse and Zebrafish (Danio rerio). It has been validated for WB.

Quick Overview for HERC4 antibody (N-Term) (ABIN2775668)

Target: HERC4 (Hect Domain and RLD 4 (HERC4))

Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HERC4 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-HERC4 Antibody

Binding Specificity: N-Term

Purpose: HERC4 Antibody - N-terminal region

Sequence: MLCWGNASFG QLGLGGIDEE IVLEPRKSDF FINKRVRDVG CGLRHTVFVL

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 93%, Rat: 100%, Zebrafish: 83%

Characteristics: This is a rabbit polyclonal antibody against HERC4. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the n terminal region of human HERC4

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP42990-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for HERC4

Target: HERC4 (Hect Domain and RLD 4 (HERC4))

Alternative Name: HERC4

Background:

Background Information: HERC4 belongs to the HERC family of ubiquitin ligases, all of which contain a HECT domain and at least 1 RCC1 (MIM 179710)-like domain (RLD). The 350-amino acid HECT domain is predicted to catalyze the formation of a thioester with ubiquitin before transferring it to a substrate, and the RLD is predicted to act as a guanine nucleotide exchange factor for small G proteins.

Gene Name: HECT and RLD domain containing E3 ubiquitin protein ligase 4

Alternative Symbols: -

Protein Name: Probable E3 ubiquitin-protein ligase HERC4

Molecular Weight: 13kDa

Gene ID: 26091

UniProt: Q5GLZ8