UBE2E1 antibody (Middle Region)

Catalog No. ABIN2775676

The Rabbit Polyclonal anti-UBE2E1 antibody is suitable to detect UBE2E1 in samples from Human, Mouse, Rabbit, Dog, Guinea Pig, Rat, Horse, Cow and Zebrafish (Danio rerio). It has been validated for WB.

Quick Overview for UBE2E1 antibody (Middle Region) (ABIN2775676)

Target: UBE2E1 (Ubiquitin-Conjugating Enzyme E2E 1 (UBE2E1))

Reactivity: Human, Mouse, Rabbit, Dog, Guinea Pig, Rat, Horse, Cow, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE2E1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-UBE2E1 Antibody

Binding Specificity: Middle Region

Purpose: UBE2E1 Antibody - middle region

Sequence: PKGDNIYEWR STILGPPGSV YEGGVFFLDI TFTPEYPFKP PKVTFRTRIY

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against UBE2E1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human UBE2E1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP43012-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for UBE2E1

Target: UBE2E1 (Ubiquitin-Conjugating Enzyme E2E 1 (UBE2E1))

Alternative Name: UBE2E1

Background:

Background Information: UBE2E1 catalyzes the covalent attachment of ubiquitin to other proteins. UBE2E1 mediates the selective degradation of short-lived and abnormal proteins.The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Gene Name: Ubiquitin-conjugating enzyme E2E 1

Alternative Symbols: UBCH6

Protein Name: Ubiquitin-conjugating enzyme E2 E1

Molecular Weight: 21kDa

Gene ID: 7324

NCBI Accession: NP_003332

UniProt: P51965

Pathways: Ubiquitin Proteasome Pathway